Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 16,115 results for: form

Showing: 2001 - 2050 of 16115 results

  9. pDEST-EGFP-Cx43ML-N1

    Plasmid
    #49860
    Purpose
    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    All internal Methionines mutated to Leucine. No s…
    Promoter
    CMV
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. pcDNA3.2-Cx43-ML-HA

    Plasmid
    #49857
    Purpose
    Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    All internal Methionines mutated to Leucine. Cont…
    Promoter
    CMV
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pcDNA3.2-Cx43-NoStart

    Plasmid
    #49853
    Purpose
    Encodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    Connexin 43 (GJA1 Human)
    Expression
    Mammalian
    Mutation
    Start codon mutated to from methionine to isoleuc…
    Promoter
    CMV
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pCHC030 (∆CBBp ∆MBH ∆SH)

    Plasmid
    #226205
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
    Use
    Synthetic Biology
    Mutation
    ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…
    Available Since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226205-plasmid-map-sequence-id-453511

    Enlarge

  21. pSB123 - pL0_rLUC-I (CDS1)

    Plasmid
    #123181
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
    Use
    Luciferase; Part for plant expression
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites; codon-optimised for plants
    Available Since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123181-plasmid-map-sequence-id-241286

    Enlarge

  22. pcDNA3.2-GJA1-20K-V5

    Plasmid
    #49859
    Purpose
    Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag
    Depositor
    Robin Shaw
    Article
    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13
    Insert
    GJA1-20k (GJA1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    GJA1-20k fragment corresponds to residues 213-382…
    Promoter
    CMV
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. CBSH3- 3m shRNA3

    Plasmid
    #160213
    Purpose
    Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Mutation
    TGTATGACCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available Since
    Oct. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    160213-plasmid-map-sequence-id-314924

    Enlarge

  29. CBSH3- 3m shRNA2

    Plasmid
    #160211
    Purpose
    Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Mutation
    TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …
    Promoter
    mouse U6 promoter
    Available Since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160211-plasmid-map-sequence-id-313962

    Enlarge

  30. CBSH3- 3m shRNA1

    Plasmid
    #160209
    Purpose
    Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).
    Depositor
    Angel de Blas
    Article
    George et al J Neurochem. 2021 May;157(4):1032-1051.
    Insert
    ARHGEF9 (Arhgef9 Rat)
    Use
    RNAi
    Mutation
    GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…
    Promoter
    mouse U6 promoter
    Available Since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160209-plasmid-map-sequence-id-313960

    Enlarge

  37. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid
    #140976
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoA-RLuc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140976-plasmid-map-sequence-id-300659

    Enlarge

  38. pcDNA5/FRT/TO-GAlphaoB-RLuc8

    Plasmid
    #140977
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoB-Rluc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140977-plasmid-map-sequence-id-300660

    Enlarge

  49. pCW57-mCherry-2A-BRD4 Iso CdelET

    Plasmid
    #137723
    Purpose
    Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)
    Depositor
    Scott Floyd
    Article
    Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi
    Insert
    BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Deleted amino acids 600-682
    Promoter
    Tight TRE promoter
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    137723-plasmid-map-sequence-id-288120

    Enlarge

Showing: 2001 - 2050 of 16115 results