We narrowed to 20,409 results for: RAN-1

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pcDNA-PU.1-ETS N236Q

Plasmid

#124615

Purpose

Chimeric ETS domain: N236Q

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a glutami…

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS N236Y

Plasmid

#124614

Purpose

Chimeric ETS domain: N236Y

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Mutation

Residues 236 altered from asparagine to a Tyrosine

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgSLC7A11/xCT-1

Plasmid

#161818

Purpose

knock out SLC7A11/xCT in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

Insert

SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 1-143

Plasmid

#108286

Purpose

Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-143

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #1

Plasmid

#179454

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shCALM2-2

Plasmid

#193700

Purpose

Constitutive lentiviral expression of CALM2 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shCALM2-3

Plasmid

#193701

Purpose

Constitutive lentiviral expression of CALM2 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3

Plasmid

#124435

Purpose

Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis Tag

Expression

Bacterial

Mutation

Residues 225 to 239 replaced with corresponding s…

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a_DmKHC[1-421]-1xGFP-6xHis

Plasmid

#196972

Purpose

Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

Depositor

Lukas Kapitein

Article

Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

Insert

Kinesin Heavy Chain (Khc Fly)

Tags

6xHis and GFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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Rat Alpha2 delta-1 pMT2

Plasmid

#58726

Purpose

expression of rat alpha2 delta-1 in mammalian cells

Depositor

Annette Dolphin

Article

Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 1-329

Plasmid

#140146

Purpose

GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 Kruppel factor (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 330 to 479

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 1-156

Plasmid

#140147

Purpose

GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 1-156 (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 157 to 479

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Wing

Plasmid

#124552

Purpose

Chimeric ETS domain: Wing of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

The 5 residues making up the "wing" in …

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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PET.SUMO eIF4G1 +MIC 1-200

Plasmid

#158792

Purpose

Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon) codon optimized

Tags

6xHIS and SUMO

Expression

Bacterial

Mutation

amino acids 1-200

Promoter

T7

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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GST-FUS ΔNLS 1-513

Plasmid

#44984

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS ΔNLS (1-513aa) (FUS Human)

Tags

GST

Expression

Bacterial

Mutation

contains amino acids 1-513

Promoter

tac

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pPHR-mCherry-PGL-1-mHP1alpha

Plasmid

#181894

Purpose

Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-PGL-1-mHP1alpha (Cbx5 Mustard Weed, Mouse)

Tags

PHR-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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