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Plasmid#123735PurposeEncodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK systemDepositorInsertERT2-fkbp-spdCa9(205-1368)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
P2_DD-HNF4A8
Plasmid#31095DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsFKBP L106P and mycExpressionMammalianMutationsplice variant 8 derived from promoter P2PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tom20-ECFP-FRB
Plasmid#210289Purposemitochondrial OM marker and "anchor" in FKBP-FRB CIDDepositorInsertTom20-ECFP-FRB
UseTagsECFP and FRBExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP-FRB-Sec61b
Plasmid#210290Purposenucleus OM marker and "anchor" for FKBP-FRB CIDDepositorInsertECFP-FRB-Sec61b
UseTagsECFP and FRBExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP-FRB-GiantinS
Plasmid#210296PurposeGolgi marker and anchor for FKBP-FRB CIDDepositorInsertECFP-FRB-GiantinS
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2AWG
Plasmid#234222PurposeHeterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP8 (PPIase FKBP8) in Escherichia coliDepositorInsert2AWG (FKBP8 Human)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-2T-FRB-W2027F
Plasmid#73341PurposePoint mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTORDepositorInsertFKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
UseTagsGST-taggedExpressionBacterialMutationFRB domain only aa2015-2114; W2027FPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB Y2105A
Plasmid#68726PurposePoint mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTORDepositorInsertFKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
UseTagsGST-taggedExpressionBacterialMutationFRB domain only aa2015-2114; Y2105APromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX_305-C-dTAG
Plasmid#91798PurposeLentiviral/gateway cloning vector for C-terminally tagging proteins of interest with the dTAG systemDepositorTypeEmpty backboneUseLentiviralTagsFKBP F36VExpressionMammalianMutationPromoterhPGKAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-AKAP95-FRB
Plasmid#138254PurposeFull-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.DepositorInsertAKAP95-FRB (AKAP8 Human)
UseTagsFRB rapamycin binding domain and HA epitopeExpressionMammalianMutationPromoterCMVAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GalNAc-T2-GFP-ESCargo(FTV)
Plasmid#140163PurposeExpresses a regulatable secretory cargo for mammalian cells and a Golgi markerDepositorInsertGalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Human, Synthetic)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
P2_DD-HNF4A8_C106R
Plasmid#31096DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsFKBP L106P and mycExpressionMammalianMutationsplice variant 8 derived from promoter P2 with zi…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB
Plasmid#26607DepositorInsertmTOR (MTOR Human)
UseTagsGSTExpressionBacterialMutationFRB domain only (aa 2015-2114)PromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR wt
Plasmid#26603PurposeMammalian expression of human flag-tagged mTorDepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR S2035T D2357E
Plasmid#26605DepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationS2035T D2357EPromoterAvailable sinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR S2035T
Plasmid#26604DepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationS2035TPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSLI-sandwich(0807600)
Plasmid#85790PurposeP. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLIDepositorInsertPF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
UseP. falciparum genomic integrationTags2xFKBP, GFPExpressionMutationPromoternoneAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB S2035I
Plasmid#26608DepositorInsertmTOR (MTOR Human)
UseTagsGSTExpressionBacterialMutationFRB domain only (aa 2015-2114); S2035IPromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag mTOR S2035T R2109A
Plasmid#26606DepositorInsertmTOR (MTOR Human)
UseTagsFlagExpressionMammalianMutationS2035T R2109APromoterAvailable sinceNov. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T FRB R2109A
Plasmid#26609DepositorInsertmTOR (MTOR Human)
UseTagsGSTExpressionBacterialMutationFRB domain only (aa 2015-2114); R2109APromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only