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pPBAD-yhjH

Plasmid

#111613

Purpose

Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

Depositor

Tim Tolker-Nielsen

Article

Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

Insert

yhjH

Expression

Bacterial

Mutation

A52V

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES2-hPAC

Plasmid

#129488

Purpose

Expresses human TMEM206

Depositor

Zhaozhu Qiu

Article

Yang et al Science. 2019 Apr 26;364(6438):395-399.

Insert

Transmembrane protein 206 (PACC1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFloat.cspA_v1

Plasmid

#133071

Purpose

Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

cspA

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

Plasmid

#115216

Purpose

Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin2Cas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

Plasmid

#115208

Purpose

Expresses PinCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PinCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Incenp wt

Plasmid

#108487

Purpose

expression of vsv-INCENP wt-mCherry

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Tags

VSV and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pWR76

Plasmid

#202776

Purpose

episomal fluorescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-p3-PE(1152)-N-term

Plasmid

#187181

Purpose

p3-PE2-N-term(1152) in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

Insert

PE2 from plasmid #132775

Use

AAV

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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UKNP1.4.2

Plasmid

#97364

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.2.2

Plasmid

#97357

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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ZJOM73

Plasmid

#133651

Purpose

Integration of CHS5-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-CHS5-mCherry (CHS5 Budding Yeast)

Tags

mCherry

Expression

Yeast

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-Craf R89L

Plasmid

#118333

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf R89L (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R89L

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pATLIC

Plasmid

#110287

Purpose

A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

Depositor

In-Geol Choi

Article

Ko et al Appl Environ Microbiol. 2012 May;78(9):3

Insert

yfaL

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-null-GFPLVA

Plasmid

#23338

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
SG3ΔENV CA E45A

Plasmid

#145783

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E45A mutation in capsid (CA E45A).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue glutamic acid 45 to alanin…

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTU424

Plasmid

#178983

Purpose

pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

TPR-CHAT

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-ZM197M-gp160

Plasmid

#123226

Purpose

Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (ZM197M) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D-5

Plasmid

#107866

Purpose

Bacterial expression of PIS, PI4K and PI4P5K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β

Tags

myc

Expression

Bacterial

Promoter

proD and proD (in operon)

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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ZJOM40

Plasmid

#133650

Purpose

Integration of SEC7-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-SEC7-DuDre (SEC7 Budding Yeast)

Tags

DuDre

Expression

Yeast

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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