19,662 results

1. beta-arrestin2 mYFP

   Plasmid

   #36917

   Depositor

   Robert Lefkowitz

   Article

   Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

   Insert

   beta-arrestin2 (Arrb2 Rat)
   

   Tags

   mYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LeGO-iG

   Plasmid

   #27358

   Depositor

   Boris Fehse

   Article

   Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

   Insert

   U6 promoter, SFFV promoter, IRES-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hAAVS1 TALEN Left

   Plasmid

   #52341

   Purpose

   TALEN vector for genomic targeting

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   AAVS1 TALEN Left
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. M7 pdnPKA-GFP

   Plasmid

   #16716

   Depositor

   Randall Moon

   Article

   Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

   Insert

   dnPKA (Prkar1a Mouse)
   

   Use

   Xenopus expression

   Tags

   GFP

   Mutation

   Dominant negative. Mutations at cAMP binding site…

   Available Since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-tMCK-ER-TurboID

   Plasmid

   #149412

   Purpose

   Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Insert

   ER-TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Promoter

   tMCK

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGP-AAV-syn-jGCaMP7f-WPRE

   Plasmid

   #104488

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

   Insert

   jGCaMP7f
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR207 SARS-CoV-2 NSP3

   Plasmid

   #141257

   Purpose

   Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pNHEJ-recX-sacB

   Plasmid

   #158718

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recX
   

   Expression

   Bacterial

   Promoter

   Pmyc

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTALYM3B15

   Plasmid

   #47878

   Purpose

   TALE-mClover against mouse major satellite sequence

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

   Insert

   TALE-mClover
   

   Use

   TALEN

   Tags

   mClover

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

    Plasmid

    #159130

    Purpose

    expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. cyto-YFP-FKBPx5

    Plasmid

    #103777

    Purpose

    expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    cyto-YFP-FKBPx5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MRGPRX2-Tango

    Plasmid

    #66440

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MRGPRX2 (MRGPRX2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. HA-p73α-pcDNA3

    Plasmid

    #22102

    Depositor

    William Kaelin

    Article

    Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

    Insert

    tumor protein p73 (TP73 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. M3-PAmcherry1

    Plasmid

    #114189

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Tags

    3xHA and PAmCherry

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-OMM-GCaMP6f

    Plasmid

    #127874

    Purpose

    Fluorescent reporter for mitochondrial outer membrane Ca2+

    Depositor

    Timothy Ryan

    Article

    Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

    Insert

    Outer membrane localized GCaMP6f
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato

    Plasmid

    #60658

    Purpose

    Flp-dependent expression of mNaChBac-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Has Service

    AAV8

    Insert

    FLEX-mNaChBac-T2A-tdTomato
    

    Use

    AAV

    Promoter

    EF1α

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUW-tetO-MCS

    Plasmid

    #84008

    Purpose

    doxycycline-inducible lentiviral vector

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJFRC150-20XUAS-IVS-Flp1::PEST

    Plasmid

    #32132

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    Flippase1::PEST
    

    Tags

    PEST

    Expression

    Insect

    Mutation

    Aspartic Acid at Amino Acid 5

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEGFP-HRas

    Plasmid

    #18662

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge