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pMOD_A0601

Plasmid

#91016

Purpose

Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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645 pCS2-etv2

Plasmid

#49005

Purpose

Mammalian Expression vector, In-situ probe template and in vitro transcription vector for zebrafish Etv2

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Invitro transcription, riboprobe template

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160-754*

Plasmid

#123212

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRW006-SM-destination-pro35S-Cas9

Plasmid

#104439

Purpose

Destination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free

Depositor

Detlef Weigel

Article

Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Plant

Promoter

35S

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_mU6

Plasmid

#102845

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert SAM_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAB085d

Plasmid

#79209

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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Hmga2 3'UTR m2 luciferase (Luc-m2)

Plasmid

#14786

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Luc-m2 (Hmga2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

m2 mutant (see article and author's map for …

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
YEpURA-CUP1-HBTubiquitin

Plasmid

#26900

Depositor

Peter Kaiser

Article

Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

Insert

HBT-tagged ubiquitin

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pAB088i

Plasmid

#79216

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-dADAM17 (#1)

Plasmid

#177259

Purpose

A knockout vector for the dog Adam17

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAB085c

Plasmid

#79208

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR221-PGK.Pro

Plasmid

#79489

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

PGK Promoter (PGK1 Human, Synthetic)

Use

Multisite gateway entry clone

Promoter

PGK

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP2-His

Plasmid

#67870

Purpose

bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP2 (PRORP2 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR221-SMAD3

Plasmid

#79507

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

SMAD3 (SMAD3 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse eIF2C3

Plasmid

#50355

Purpose

Mouse eIF2C3 cDNA clone

Depositor

Kumiko Ui-Tei

Article

Doi et al Curr Biol. 2003 Jan 8;13(1):41-6.

Insert

eIF2C3 (Ago3 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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Double UP mNeon to mScarlet Rac1-N17

Plasmid

#125137

Purpose

Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

mNeon/mScarletP2A Rac1-N17

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet. 17th AA in Rac1 ch…

Promoter

CAG

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159847

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pQsupR-Mi2b

Plasmid

#15379

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

short hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)

Use

RNAi and Retroviral

Available Since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pMOD_C2813

Plasmid

#91092

Purpose

Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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