We narrowed to 17,806 results for: Met;

1. mOrange2-Golgi-7

   Plasmid

   #57959

   Purpose

   Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Golgi (B4GALT1 Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   aa 1-82 of NM_001497.3

   Promoter

   CMV

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

2. MERS-coV-NSP3-c001

   Plasmid

   #173085

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   D1109-D1275, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCardinal-Tensin1-N-18

   Plasmid

   #56175

   Purpose

   Localization: Focal Adhesions, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Tensin1 (TNS1 Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

   Plasmid

   #35508

   Purpose

   AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   E123A

   Promoter

   Ef1a

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_VNp-FGF21

   Plasmid

   #182407

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-fgf21 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-fgf21 (FGF21 Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

   Plasmid

   #98932

   Purpose

   Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   iGluSnFr
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. mIFP-H2B-6

   Plasmid

   #56223

   Purpose

   Localization: Nucleus/Histones, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mIFP

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pAAV-CAGGS-Flex/3'USS(Luc)-GFP

   Plasmid

   #197887

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xEcoLeuT(CUA)_EF1_AnapRS

   Plasmid

   #174894

   Purpose

   AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   AnapRS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid

    #86610

    Purpose

    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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