We narrowed to 9,126 results for: control

1. pDECKO_mCherry_Rat_cebp-alfa

   Plasmid

   #140098

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDECKO_mCherry_SPI1

   Plasmid

   #140099

   Purpose

   CRISPR-Cas9 library validation (positive control)

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-SV40P-Tet1 enhancer E

   Plasmid

   #63880

   Purpose

   Luciferase reporter plasmid used to study transcriptional control of murine Tet1

   Depositor

   Kian Peng Koh

   Article

   Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

   Insert

   Tet1 enhancer fragment E
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a_POU5F1_P2A_Hygro_Barcode

   Plasmid

   #120474

   Purpose

   Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. shCTL-mlpx-puro

   Plasmid

   #65232

   Purpose

   encodes a non-targeting control shRNA

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   non-coding shRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

   Plasmid

   #157699

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pJMP1341

   Plasmid

   #119272

   Purpose

   control sgRNA in cells lacking rfp

   Depositor

   Oren Rosenberg, Jason Peters, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

   Plasmid

   #157692

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pcDNA5-FRT-TO-FH-Nsp8

   Plasmid

   #157695

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp8 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. LV_EF1a_Caspase8(C360A)-P2A-Hygro_Barcode

    Plasmid

    #170217

    Purpose

    Barcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CASP8 (CASP8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    C360A

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. JA524

    Plasmid

    #49938

    Purpose

    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in EGFP for use as a control

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

    Insert

    Tet1CD GFP (TET1 Human)
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5-FRT-TO-FH-Nsp15

    Plasmid

    #157721

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp15 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

    Plasmid

    #170225

    Purpose

    Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    ER (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Y537S

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-EF1a-Ly6G-miR30

    Plasmid

    #163358

    Purpose

    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-FRT-TO-FH-Nsp7

    Plasmid

    #157689

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid

    #157693

    Purpose

    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA5-FRT-TO-Nsp15-HF

    Plasmid

    #157722

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp15-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMY-Ly6G-Rluc_miR

    Plasmid

    #163339

    Purpose

    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    Ly-6G (Ly6g Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 PA-NL

    Plasmid

    #113445

    Purpose

    ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63886

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human SYN1

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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