We narrowed to 13,661 results for: cDNA

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SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#174666

Purpose

Doxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a-3xFLAG (Arid3a Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-hHelios-IRES-GFP

Plasmid

#74047

Purpose

retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Helios (IKZF2) (IKZF2 )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

encodes shorter version of human Helios

Promoter

pMSCV-LTRs

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK3-CR

Plasmid

#229422

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK3-CR (MARK3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

silent mutation CRISPR resistant

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FLAG-hIKAROS-IRES-GFP

Plasmid

#74046

Purpose

retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Ikaros (IKZF1) (IKZF1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

pMSCV-LTRs

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNTGFBR2-HA

Plasmid

#130888

Purpose

Expresses dominant negative mutant human TGFBR2

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

TGFBR2 (TGFBR2 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHCD1C

Plasmid

#102954

Purpose

gene cloning of human CD1C

Depositor

Gennaro De Libero, Lucia Mori

Article

Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

Insert

CD1C cDNA (CD1C Human)

Use

Cloning

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-MYO5C

Plasmid

#135404

Purpose

Expresses GFP-tagged human MYO5C in mammalian cells.

Depositor

Richard Cheney

Article

Jacobs et al Mol Biol Cell. 2009 Nov;20(21):4471-88.

Insert

MYO5C (MYO5C Human)

Tags

EGFP

Expression

Mammalian

Mutation

Relative to the GenBank MYO5C cDNA sequence repor…

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-OGDH_wobble

Plasmid

#110938

Purpose

OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

OGDH wobble cDNA (OGDH Human)

Use

Lentiviral

Mutation

15 silent wobble mutations corresponding to three…

Promoter

SV40

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-YFP

Plasmid

#55781

Purpose

This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

alpha-s-EE YFP (Gnas Rat)

Tags

EYFP flanked by SGGGS on each side was inserted i…

Expression

Mammalian

Mutation

Met was substituted for Gln69 in EYFP (Clontech).…

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.ARID3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169314

Purpose

Doxycycline-inducible expression of human ARID3A cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

ARID3A (ARID3A Human)

Use

Lentiviral

Tags

IRES-dTomato

Promoter

TRE

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-6xHis-SUMO1

Plasmid

#164939

Purpose

Expresses 6xHis tagged human Small Ubiquitin Like Modifier 1 (SUMO1) wild type cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Small Ubiquitin Like Modifier 1 (SUMO1 Human)

Use

Lentiviral

Tags

6XHIS

Promoter

CMV

Available Since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A Δ820-870-pBabe-Puro

Plasmid

#25949

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α bHLH* P405A/P531A Δ820-870-pBabe-Puro

Plasmid

#25957

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A Δ450-572/Δ820-870-pBabe-Puro

Plasmid

#25955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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HA-YAP-wt

Plasmid

#229412

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

YAP-wt (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryCXCR4-W

Plasmid

#200102

Purpose

Lentiviral vector expressing mCherry fused with CXCR4 cDNA at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

CXCR4 (CXCR4 Human)

Use

CRISPR and Lentiviral

Mutation

silent mutations (c.180C>T, 438G>A, 495C>…

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-Nkx2-1*/Neo

Plasmid

#31272

Depositor

Tyler Jacks, Monte Winslow

Article

Winslow et al Nature. 2011 May 5. 473(7345):101-4.

Insert

Nkx2-1* (Nkx2-1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

A shNkx2-1 insensitive Nkx2-1 cDNA was created by…

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-HA-FLAG_Ubc9_WT

Plasmid

#164936

Purpose

Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-SAE1_sh1

Plasmid

#168984

Purpose

Expresses RFP cDNA and SAE1 shRNA 1 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

SUMO1 Activating Enzyme Subunit 1 (SAE1 Human)

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20_SLC38A2_wt

Plasmid

#156182

Purpose

Dox-inducible expression of sgRNA-resistant SLC38A2 wild-type cDNA.

Depositor

Alec Kimmelman

Article

Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

Insert

SLC38A2 (SLC38A2 Human)

Expression

Mammalian

Mutation

Silent mutations to prevent targeting by sgRNA: T…

Promoter

TRE

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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