We narrowed to 2,364 results for: EFS
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pHH0103_EEF1A1_GTP-EFTU-D2
Plasmid#109811PurposeProtein expression and purification of EEF1A1_GTP-EFTU-D2DepositorInsertEEF1A1_GTP-EFTU-D2 (EEF1A1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p23-mNeongreen-DDX21 HGV
Plasmid#179230Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreenExpressionMammalianMutationD338H, E339GPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 DAT
Plasmid#179229Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmNeongreenExpressionMammalianMutationG234D, K235APromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNMT3ACD-CRY2-EGFP
Plasmid#82556PurposeEncodes DMNT3 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-92a-1-5p
Plasmid#103750PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-92a-1-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-92a-1-5p target (MIR92A1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized
Plasmid#172346PurposeExpression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLINK cyclin T1 (1-280) myc (all 200) (P#575)
Plasmid#14610DepositorInsertcyclin T1 (1-280) (all 200) (CCNT1 Human)
UseTagsmycExpressionMammalianMutationC198A, C200A, H202A, C205A. Active in 3T3 cells.PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Tet-Off-FLEX-DEST (JDW 1186)
Plasmid#229820PurposeAn AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTADepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)
Plasmid#229824PurposeA PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tetDepositorInsertLuciferase-P2A-H2A-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRpuro-sgSEC24C #2
Plasmid#231564PurposepLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24C (#2) (Puromycin selection marker)DepositorInsertsgSEC24C #2 (SEC24C Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF-1aAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRpuro-sgSEC24C #1
Plasmid#231563PurposepLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24C (#1) (Puromycin selection marker)DepositorInsertsgSEC24C #1 (SEC24C Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF-1aAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRpuro-sgSEC24D
Plasmid#231565PurposepLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24D (Puromycin selection marker)DepositorInsertsgSEC24D (SEC24D Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF-1aAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
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V5-14-3-3-e
Plasmid#229426PurposeLentiviral or overpexression of cDNADepositorInsertYWHAE (YWHAE Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEFSAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
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HA-MST2-S444A
Plasmid#229420PurposeLentiviral or overpexression of cDNADepositorInsertMST2-S444A (STK3 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosite mutant (S444A)PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pTMB303_ZFcharm_Kv1_Prnp_SMM
Plasmid#220841PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; single mismatch self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LbCas12a-8xNLS
Plasmid#209020PurposeLentiviral vector expressing human codon-optimized LbCas12a with 8xNLSDepositorInsertLbCas12a-8xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-hMICU1EF
Plasmid#199180PurposeThis plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.DepositorInsertMICU1 (MICU1 Human)
UseTagsHis6 and MBP, Thrombin site, TEV siteExpressionMutationD421A, E432K, F433W, F453WPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1548
Plasmid#143292PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTAZ (TAFAZZIN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1550
Plasmid#142808PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTAZ (TAFAZZIN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1549
Plasmid#142807PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTAZ (TAFAZZIN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
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p23-mRuby3-DDX21 HGV
Plasmid#179233Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
UseTagsmRuby3ExpressionMammalianMutationD338H, E339GPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g3 (BB36)
Plasmid#139454PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Neo-NCOA7g2 (BB35)
Plasmid#139453PurposeLentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable markerDepositorInsertNCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1a / U6Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113701PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113699PurposeDox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX
Plasmid#102787PurposeExpresses the Reverse Transcriptase geneDepositorInsertreverse transcription xenopolymerase
UseTagsExpressionBacterialMutationPromoterT7 promoter + lac operatorAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_RTX_(exo-)
Plasmid#102786PurposeExpresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)DepositorInsertreverse transcription xenopolymerase with 3'-5' deficient exonuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP71-hCD155
Plasmid#118630Purposehuman CD155 coding sequence subcloned into pMP71 retroviral plasmidDepositorInsertCD155 (PVR Human)
UseTagsExpressionMammalianMutationPromoterLTRAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only
