Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 5,876 results for: nls

Showing: 2051 - 2100 of 5876 results

  1. DsRed2-Nucleus-4

    Plasmid
    #55839
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    DsRed2-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. mPlum-Nucleus-7

    Plasmid
    #55991
    Purpose
    Localization: Nucleus, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    mPlum-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. mRuby-Nucleus-7

    Plasmid
    #55875
    Purpose
    Localization: Nucleus, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    mRuby-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. Dronpa-Nucleus-9

    Plasmid
    #57290
    Purpose
    Localization: Nucleus, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    Dronpa-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57290-plasmid-map-sequence-id-183119

    Enlarge

  5. HcRed1-Nucleus-7

    Plasmid
    #55843
    Purpose
    Localization: Nucleus, Excitation: 588, Emission: 618
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    HcRed1-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. mTagBFP-Nucleus-7

    Plasmid
    #55265
    Purpose
    Localization: Nuclear Localization, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    mTagBFP-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55265-plasmid-map-sequence-id-475376

    Enlarge

  8. Dendra2-Nucleus-7

    Plasmid
    #57734
    Purpose
    Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    Dendra2-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. Kaede-Nucleus-9

    Plasmid
    #57319
    Purpose
    Localization: Nucleus, Excitation: 508 / 572, Emission: 518 / 580
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    Kaede-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. tdEos-Nucleus-7

    Plasmid
    #57650
    Purpose
    Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    SV40 T-Antigen Nuclear Localization Signal (NLS)
    Use
    Tags
    tdEos-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pYPQ166-SpRY

    Plasmid
    #161520
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter
    Depositor
    Yiping Qi
    Article
    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41
    Insert
    zSpRYCas9
    Use
    CRISPR; Gateway compatible zsprycas9 entry clone
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    161520-plasmid-map-sequence-id-325640

    Enlarge

  15. pHSN401

    Plasmid
    #50588
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50588-plasmid-map-sequence-id-75660

    Enlarge

  16. pBUN411

    Plasmid
    #50581
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50581-plasmid-map-sequence-id-311045

    Enlarge

  19. pCSN075

    Plasmid
    #166732
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166732-plasmid-map-sequence-id-332206

    Enlarge

  20. pCSN074

    Plasmid
    #166731
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166731-plasmid-map-sequence-id-332203

    Enlarge

  21. PRPF4.001.522.K45A.pc4

    Plasmid
    #137648
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-K45A-pc4
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137648-plasmid-map-sequence-id-381373

    Enlarge

  22. PRPF4.001.522.Y32A.pc4

    Plasmid
    #137646
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-Y32A-pc4
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137646-plasmid-map-sequence-id-381367

    Enlarge

  25. pDONR221_zCas9

    Plasmid
    #213913
    Purpose
    Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Sébastien Mongrand
    Article
    Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi
    Insert
    zCas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213913-plasmid-map-sequence-id-437263

    Enlarge

  29. pCSN071

    Plasmid
    #166729
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166729-plasmid-map-sequence-id-332207

    Enlarge

  30. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

    Enlarge

  31. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71288-plasmid-map-sequence-id-166343

    Enlarge

  32. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

    Enlarge

  33. pCSKaedenERT(#100)

    Plasmid
    #184064
    Purpose
    cyclofen-inducible Kaede nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    Kaede-nls-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    July 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184064-plasmid-map-sequence-id-365948

    Enlarge

  34. pMP597

    Plasmid
    #134999
    Purpose
    Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC methylation to ZF binding sites. pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134999-plasmid-map-sequence-id-267926

    Enlarge

  35. pYPQ166-iSpyMac

    Plasmid
    #149553
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    iSpyMacCas9
    Use
    CRISPR; Gateway compatible zispymaccas9 entry clo…
    Tags
    3X FLAG, NLS and NLS
    Expression
    Plant
    Mutation
    R221K, N394K, Protospacer interacting domain form…
    Promoter
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149553-plasmid-map-sequence-id-306139

    Enlarge

  40. HBT_pcoCASphi_version1

    Plasmid
    #197931
    Purpose
    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal
    Depositor
    Steven Jacobsen
    Article
    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12
    Insert
    pcoCasphi-2
    Use
    CRISPR
    Tags
    FLAG tag and SV40 NLS and nucleoplasmin NLS
    Expression
    Plant
    Mutation
    Promoter
    35SPPDK promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    197931-plasmid-map-sequence-id-388007

    Enlarge

  42. pTriEx-HTNC

    Plasmid
    #13763
    Purpose
    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.
    Depositor
    Klaus Rajewsky
    Article
    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99
    Insert
    His-TAT-NLS-Cre
    Use
    Baculovirus
    Tags
    His Tag, NLS, and TAT
    Expression
    Bacterial
    Mutation
    Promoter
    p10 promoter
    Available since
    April 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13763-plasmid-map-sequence-id-364424

    Enlarge

  46. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid
    #117070
    Purpose
    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
    Depositor
    Kim Failor
    Inserts
    Cas9
    SacB
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117070-plasmid-map-sequence-id-231696

    Enlarge

  47. BII-tdT-inv-GFP

    Plasmid
    #133393
    Purpose
    Piggybac vector for bidirectional promoter reporter assay
    Depositor
    Blair Madison
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    No promoter
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133393-plasmid-map-sequence-id-268036

    Enlarge

  48. BII-ChPtW-smURFP

    Plasmid
    #133399
    Purpose
    Piggybac vector for constitutive expression of smURFP-NLS
    Depositor
    Blair Madison
    Insert
    smURFP-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133399-plasmid-map-sequence-id-268584

    Enlarge

  49. pT22iuasvApoWtEos3N(#486)

    Plasmid
    #184107
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    Expression
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    4xUAS
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184107-plasmid-map-sequence-id-363989

    Enlarge

  50. BII-ChPtW-iRFP670

    Plasmid
    #133397
    Purpose
    Piggybac vector for constitutive expression of iRFP670-NLS
    Depositor
    Blair Madison
    Insert
    iRFP-670-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133397-plasmid-map-sequence-id-269430

    Enlarge

Showing: 2051 - 2100 of 5876 results