We narrowed to 1,197 results for: Aspa

1. pET28a-MBP-CBX1-eReader

   Plasmid

   #135089

   Purpose

   Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

   Depositor

   Marcey Waters

   Article

   Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

   Insert

   CBX1 chromobox protein 1, engineered
   

   Use

   Tags

   6xhis-tag and MBP

   Expression

   Bacterial

   Mutation

   lysine 43 mutated to alanine; aspartic acid 59 mu…

   Promoter

   T7

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2XMyc-LRRK2-D1994A

   Plasmid

   #25368

   Depositor

   Mark Cookson

   Article

   J Biol Chem. 2008 Jun 13;283(24):16906-1

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   2XMyc

   Expression

   Mammalian

   Mutation

   Aspartate 1994 mutated to Alanine

   Promoter

   Available since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. DsRed-rab9 DN

   Plasmid

   #12676

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB9 (Homo sapiens) (RAB9A Human)
   

   Use

   Tags

   DsRed and HA

   Expression

   Mammalian

   Mutation

   changed serine 21 to asparagine

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. SG3ΔENV RT D185A

   Plasmid

   #145782

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

   Depositor

   Wesley Sundquist

   Article

   Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

   Insert

   Reverse Transcriptase
   

   Use

   Lentiviral and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed Reverse Transcriptase Aspartic Acid 185 t…

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b/bPanK

   Plasmid

   #73619

   Purpose

   Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

   Depositor

   T. J. Kappock, Suzanne Jackowski

   Article

   Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

   Insert

   pantothenate kinase (coaA Escherichia coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   relative to MG1655 sequence, changes glutamate-5 …

   Promoter

   T7

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL WS1.6 WASp

   Plasmid

   #36250

   Depositor

   David Rawlings

   Article

   Astrakhan et al Blood. 2012 Mar 19.

   Insert

   WASp (WAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   WS1.6

   Available since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. AlkB-D135T

   Plasmid

   #160433

   Purpose

   A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr

   Depositor

   Tao Pan

   Article

   Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30

   Insert

   E. coli AlkB
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 135 to Threonine; deleted t…

   Promoter

   T7

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-Merlin S518D

   Plasmid

   #84295

   Purpose

   Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Merlin (NF2) (NF2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S518D

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP-Cdc42(N17)

   Plasmid

   #11400

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   cell division cycle 42 (CDC42 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Obtained with Threonine 17 changed to Asparagine.

   Promoter

   Available since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-IP-EGFP-ULK1(K46N)

    Plasmid

    #38197

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Lysine 46 to Asparagine

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pTol1:uas-myc-act-map2k2b-2a-tdTomato

    Plasmid

    #104721

    Purpose

    UAS-driven expression of activated MEK

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    activated map2k2b (map2k2b Zebrafish)
    

    Use

    Zebrafish transposon

    Tags

    2A-tdTomato and myc tag

    Expression

    Mutation

    serines 221 and 225 to aspartic acid

    Promoter

    10xUAS

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Opto-casp9_VfAU1-LOV_casp9

    Plasmid

    #131220

    Purpose

    Expresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9)

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    Opto-casp9 (VfAU1-LOV_casp9)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-Rac1(N17)

    Plasmid

    #11395

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 mutated to Asparagine.

    Promoter

    Available since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-mNINJ1-D53A-GFP

    Plasmid

    #208778

    Purpose

    MSCV vector expressing D53A mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Changed aspartic acid 53 to alanine (D53A)

    Promoter

    Available since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEos2-CD9-10

    Plasmid

    #57356

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3/mArf3(T31N)-HA

    Plasmid

    #79416

    Purpose

    Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

    Insert

    Arf3 (Arf3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 31 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJCC_075 AsCas12a D908A

    Plasmid

    #171668

    Purpose

    For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    AsCas12a D908A
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed aspartate 908 to alanine

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-75-2

    Plasmid

    #73036

    Purpose

    Express an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cells

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Insert

    p75-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mature Human NT-3 gene exchanged Aspartic 15 to a…

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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