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pcDNA3/mArf3(T31N)-HA

Plasmid

#79416

Purpose

Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-75-2

Plasmid

#73036

Purpose

Express an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cells

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Insert

p75-2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Mature Human NT-3 gene exchanged Aspartic 15 to a…

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCT

Plasmid

#60620

Purpose

Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

Depositor

Huimin Zhao

Article

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98N-pBabe-puro

Plasmid

#19236

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 98 to Asparagin

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pMTP130 (pTA106_TnsABC(Tn6900))

Plasmid

#164261

Purpose

Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Insert

tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1

Use

Tags

Expression

Bacterial

Mutation

(tnsA) Changed Alanine 125 to Aspartic acid

Promoter

Lactose

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBS U6 WASp RNAi

Plasmid

#11775

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WASp RNAi (WAS Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
TPC2-D276K-G-GECO1.2

Plasmid

#207142

Purpose

Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPC2N2 (TPCN2 Human)

Use

Tags

G-GECO1.2

Expression

Mammalian

Mutation

Changed Aspartate 276 to Lysine

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y112N-pBabe-puro

Plasmid

#19238

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 112 to Asparagin

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

Plasmid

#122067

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Use

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJCC_058 FnCas12a D917A

Plasmid

#171667

Purpose

For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a D917A

Use

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Mutation

changed aspartate 917 to alanine

Promoter

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-CD151-7

Plasmid

#57358

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD151 (CD151 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-MBP-(CBX1)3-eReader

Plasmid

#135090

Purpose

Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered x3

Use

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUHG10.3-TetO-Rs1

Plasmid

#16313

Depositor

Bruce Conklin

Article

Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

Insert

h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Added FLAG tag; Changed Aspartic Acid 100 to Alan…

Promoter

Available since

Jan. 3, 2008

Availability

Academic Institutions and Nonprofits only

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NmMetQ N238A

Plasmid

#129648

Purpose

Expresses N238A mutation in the mature metQ gene from Neisseria meningitides, without the signal sequence

Depositor

Douglas Rees

Article

Nguyen et al Protein Sci. 2019 Jul 26. doi: 10.1002/p

Insert

metQ Gene

Use

Tags

10x Histidine Tag, Enterokinase, and pelB signal …

Expression

Bacterial

Mutation

Changed Asparagine 238 to Alanine

Promoter

T7 Promoter

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTag002 DA-MBP

Plasmid

#102832

Purpose

Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyTag002 DA-MBP

Use

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Reactive aspartic acid has been mutated to an ala…

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET30b GluBP600n

Plasmid

#119835

Purpose

Production of recombinant GluBP600n high affinity protein in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

GluBP600n

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Bmf (S74D)

Plasmid

#24259

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (S87D) (BMF Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 74 to Aspartic Acid

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL (T56D)

Plasmid

#24237

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (T56D) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL (S58D)

Plasmid

#24239

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S58D) (BCL2L11 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 58 to aspartic acid

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only