We narrowed to 2,319 results for: fas

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pBluescript-IC3-FLAG-Tev-ZZ

Plasmid

#170311

Purpose

Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

Depositor

Andrew Carter

Publication

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Outer Dynein Arm Intermediate Chain 3

Tags

FLAG, ZZ

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-Nb127D01-GFP

Plasmid

#171577

Purpose

Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Publication

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-Nb127D01-GFP (CXCR2 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C14A

Plasmid

#89977

Purpose

Expresses catalytic inactive GFP-tagged ataxin-3

Depositor

Nico Dantuma

Publication

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 14 to Alanine

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 U6-SaAi9L-U6-SaAi9R

Plasmid

#78604

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

Depositor

Amy Wagers

Publication

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

gRNAs for SaCas9 targeting Ai9 locus

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only
pBBR1.cas9-NT

Plasmid

#197871

Purpose

Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

Depositor

Baojun Wang

Publication

Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

Inserts

trancCRNA
Cas9
crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)

Expression

Bacterial

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCCLc-U6-shHMGA2.2-PGK-dTomato

Plasmid

#89605

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Publication

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE

Plasmid

#89607

Purpose

Expression of LIN28A in human cells

Depositor

Fernando Fierro

Publication

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

LIN28A (LIN28A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pNCS-mGold2s

Plasmid

#231765

Purpose

Expression of mGold2s in bacterial cells

Depositor

Francois St-Pierre

Publication

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Bacterial

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Synthetic promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMX-DivisionRecorder

Plasmid

#179446

Purpose

Retroviral expression vector containing the DivisionRecorder cassette

Depositor

Ton Schumacher

Publication

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

GFP-P2A-511-CrePI-511

Use

Retroviral

Promoter

LTR

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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shERK2b-mlpx-puro

Plasmid

#65230

Purpose

encodes a shRNA against ERK2

Depositor

Gerardo Ferbeyre

Publication

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

shRNA against ERK2 (MAPK1 Human)

Expression

Mammalian

Promoter

PGK

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-NbVHH05-GFP

Plasmid

#171576

Purpose

Expresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Publication

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-NbVHH05-GFP (UBE2J1 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-Giantin_C1

Plasmid

#189773

Purpose

Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-Giantin

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mVenus-C1

Plasmid

#54651

Purpose

Localization: C1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

mVenus

Expression

Mammalian

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles
pCDHblast-flx-sORF-Katushka

Plasmid

#179457

Purpose

Lentiviral vector encoding a CRE inducible Katushka gene

Depositor

Ton Schumacher

Publication

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

loxP-shuffledORF-loxP-Katushka

Use

Lentiviral

Promoter

CMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-nonstop

Plasmid

#226406

Purpose

Expresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.

Depositor

Nico Dantuma

Publication

Xu et al Commun Biol. 2024 Oct 8;7(1):1282. doi:

Insert

EGFP without stop codon

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBluescript-Q22YU3-KO

Plasmid

#170313

Purpose

Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22YU3 with Paromomycin selection

Depositor

Andrew Carter

Publication

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22YU3 knockout

Use

Tetrahymena somatic gene knockout vector

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript-Q22MS1-KO

Plasmid

#170312

Purpose

Plasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selection

Depositor

Andrew Carter

Publication

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Q22MS1 knockout

Use

Tetrahymena somatic gene knockout vector

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10

Plasmid

#104618

Purpose

Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

hsp70-Syn21-FLAG-mCerulean-P10

Tags

FLAG

Expression

Insect

Promoter

hsp70

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC81-3xVAS4-IVS-Syn21-GFP-P10

Plasmid

#104615

Purpose

Expresses GFP upon binding of TALE4 to VAS4

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

3xVAS4

Expression

Insect

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJFRC81-3xVAS1-Syn21-Citrine-HA-P10

Plasmid

#104616

Purpose

Expresses HA tagged Citrine upon binding of TALE1 to VAS1

Depositor

Tudor Fulga

Publication

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

Syn21-Citrine-HA-P10-MhcSD

Tags

HA

Expression

Insect

Promoter

hsp70

Available since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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