We narrowed to 6,730 results for: tac

1. pGEX3X-RanBP2dFG

   Plasmid

   #53120

   Purpose

   E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

   Depositor

   Frauke Melchior

   Article

   Pichler et al Cell. 2002 Jan 11;108(1):109-20.

   Insert

   RanBP2 fragment (RANBP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   fragment (amino acids 2553-2838)

   Promoter

   tac

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. SRpHi4

   Plasmid

   #102544

   Purpose

   Bacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purification

   Depositor

   Stefan Hell

   Article

   Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

   Insert

   EYFP-EGF
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX6P1-A20 (OTU, aa 1-366)

   Plasmid

   #61584

   Purpose

   Expresses human A20 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Komander et al Biochem J. 2008 Jan 1;409(1):77-85.

   Insert

   A20 (TNFAIP3 Human)
   

   Use

   Tags

   GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 367-790.

   Promoter

   tac

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. SRpHi2

   Plasmid

   #102542

   Purpose

   Bacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purification

   Depositor

   Stefan Hell

   Article

   Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

   Insert

   Tat-EGFP
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SRpHi3

   Plasmid

   #102543

   Purpose

   Bacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purification

   Depositor

   Stefan Hell

   Article

   Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

   Insert

   Tat-sepHluorin
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Dhh1

   Plasmid

   #201412

   Purpose

   Bacterial protein expression of Dhh1

   Depositor

   Michael Rosen

   Article

   Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Dhh1 (DHH1 Budding Yeast)
   

   Use

   Tags

   MBP, TEV and TEV, 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SRpHi1

   Plasmid

   #102541

   Purpose

   Bacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purification

   Depositor

   Stefan Hell

   Article

   Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

   Insert

   Tat-EYFP
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FRB-polySH33-EGFP

   Plasmid

   #175792

   Purpose

   Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FRB-polySH35

   Plasmid

   #175234

   Purpose

   Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FRB-polySH33

    Plasmid

    #175233

    Purpose

    Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-PML peptide

    Plasmid

    #175246

    Purpose

    Bacterial expression and purification, low affinity SUMOylation substrate

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    PML (PML Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MBP-TEV-VMA Model vector

    Plasmid

    #86590

    Purpose

    This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    VMA intein
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Original SapI site from pMal was mutated out

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGEX2TK-YAP-WW1

    Plasmid

    #104305

    Purpose

    bacterial expression of WW domain 1 of YAP; binds PPxY where Y is not phosphorylated

    Depositor

    Marius Sudol

    Insert

    YAP WW1 domain (YAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    WW domain 1

    Promoter

    tac

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-hXRCC1-K176R

    Plasmid

    #52275

    Purpose

    Bacterial expression of human XRCC1-K176R with N-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K176R, SUMO acceptor site mutant

    Promoter

    tac

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJUMP19-L3S1P51_CT

    Plasmid

    #127047

    Purpose

    Basic Part CT- C-terminus + Terminator; L3S1P51. C-termini STOP + terminator (synthetic)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part L3S1P51_CT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GST-PRMT7-ΔC

    Plasmid

    #34695

    Depositor

    Steven Clarke

    Article

    Miranda et al J Biol Chem. 2004 May 28;279(22):22902-7

    Insert

    PRMT7 (PRMT7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 1-378; and N375K was introduced with …

    Promoter

    tac

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

17. Edc3

    Plasmid

    #201413

    Purpose

    Bacterial protein expression of Edc3

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Edc3 (EDC3 Budding Yeast)
    

    Use

    Tags

    MBP, TEV and TEV, 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Pat1

    Plasmid

    #201414

    Purpose

    Bacterial protein expression of Pat1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Pat1 (PAT1 Budding Yeast)
    

    Use

    Tags

    MBP, TEV and TEV, 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dcp2

    Plasmid

    #201411

    Purpose

    Bacterial protein expression of Dcp2

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    MBP, TEV and TEV, 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dcp1

    Plasmid

    #201410

    Purpose

    Bacterial protein expression of Dcp1

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp1 (DCP1 Budding Yeast)
    

    Use

    Tags

    MBP, TEV and TEV, 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge