166,289 results

1. pcDNA3-Venus-173-N-ORAI1

   Plasmid

   #87618

   Purpose

   N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Venus173-N-ORAI1 (ORAI1 Human, Synthetic)
   

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF hPGK mc2 IRES blast

   Plasmid

   #206239

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190033

   Purpose

   vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13X
   

   Expression

   Mammalian

   Mutation

   Y672A,Y676A

   Promoter

   CBh, SV40, hU6

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-Tau-nYFP

   Plasmid

   #92204

   Purpose

   Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   MAPT (MAPT Human)
   

   Use

   Retroviral

   Tags

   YFP(1-155)

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBT29-splitD

   Plasmid

   #122599

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LSB-hsa-miR-30d-5p

   Plasmid

   #103417

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-30d-5p target (MIR30D Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer-FRA1

   Plasmid

   #192267

   Purpose

   Doxycycline-inducible expression of murine FRA1 (Fosl1)

   Depositor

   Günter Schneider

   Article

   Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

   Insert

   Fosl1 (Fosl1 Mouse)
   

   Use

   Lentiviral; Inducible expression

   Expression

   Mammalian

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-PILRA-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156577

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   PILRA (PIGR Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT29-splitC

   Plasmid

   #122598

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Expression

   Bacterial

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-RFP-IKZF1-sh3

    Plasmid

    #69042

    Purpose

    Lentiviral expression of IKZF1 shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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