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169,691 results

Showing: 4191 - 4200 of 169691 results

  1. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid
    #47550
    Purpose
    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    ttTi5605 sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47550-plasmid-map-sequence-id-67865

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  2. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid
    #50476
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50476-plasmid-map-sequence-id-171073

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  5. GPRC5C-Tango

    Plasmid
    #66384
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPRC5C (GPRC5C Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pclbw-eGFPomp25

    Plasmid
    #58424
    Purpose
    Mammalian expression of GFP targeted to the mitochondrial outer membrane
    Depositor
    David Chan
    Article
    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi
    Insert
    eGFP-omp25 (Synj2bp Mouse)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    contains aa's 109-145 of mouse omp25
    Promoter
    CMV/b-actin
    Available Since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. pRF0G-IodoY

    Plasmid
    #113645
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    IodoYRS and tRNA gene
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113645-plasmid-map-sequence-id-217127

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Showing: 4191 - 4200 of 169691 results