We narrowed to 9,182 results for: Pol

1. pMuLE ENTR 7SK L1-L4

   Plasmid

   #62110

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter for shRNA expression. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Type

   Empty backbone

   Use

   RNAi; Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK RagA-RagC(Q120L)

   Plasmid

   #99701

   Purpose

   Bacterial expression of RagA-RagC(Q120L)

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   Q120L

   Promoter

   T7

   Available Since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTAtUbq3 (GB0219)

   Plasmid

   #68192

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq3 terminator
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15b+PCNA3 R112C/T180C

   Plasmid

   #66182

   Purpose

   Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C, T180C

   Promoter

   T7 promoter

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b+PCNA2 E105C/L171C

   Plasmid

   #66178

   Purpose

   Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 2
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E105C, L171C

   Promoter

   T7 promoter

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. D10 pAcGP67a

   Plasmid

   #41812

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   D10 Super-2 (IL2 Human)
   

   Tags

   8x His

   Expression

   Insect

   Promoter

   Polyhedron

   Available Since

   Jan. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pmRNA-iEMCV-EGFP-A120

   Plasmid

   #225905

   Purpose

   In vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.

   Depositor

   Tom Brown

   Article

   Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

   Inserts

   A-inserted T7 class III Φ6.5 promoter
   EMCV IRES
   EGFP
   2x β-globin UTR
   poly(A) tail
   

   Use

   Synthetic Biology; Mrna preparation

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmRNA-iHCV-EGFP-A120

   Plasmid

   #225906

   Purpose

   In vitro transcription of cap-independently (HCV IRES) translated mRNA encoding EGFP.

   Depositor

   Tom Brown

   Article

   Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

   Inserts

   A-inserted T7 class III Φ6.5 promoter
   HCV IRES
   EGFP
   2x β-globin UTR
   poly(A) tail
   

   Use

   Synthetic Biology; Mrna preparation

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-DIO-Varnam2-WPRE

   Plasmid

   #240055

   Purpose

   Cre dependent Red fluorescent, negative response-polarity voltage indicator for mammalian expression

   Depositor

   Mark Schnitzer

   Article

   Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

   Insert

   VARNAM2
   

   Use

   AAV

   Promoter

   Ef1a

   Available Since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. IMPT-11371

    Plasmid

    #236191

    Purpose

    Express GPR119 in insect cells

    Depositor

    Vadim Cherezov

    Article

    Kim et al Structure. 2024 Dec 5;32(12):2206-2219.e

    Insert

    GPR119 (GPR119 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEW107

    Plasmid

    #232796

    Purpose

    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
    

    Tags

    His6-3xFLAG, Twinstrep

    Expression

    Insect

    Mutation

    ySet1(762-1080)

    Promoter

    polyhedrin promoter

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210734

    Purpose

    Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Frodo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRII-TOPO-dgat1a in situ probe

    Plasmid

    #232137

    Purpose

    zebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    dgat1a (dgat1a Zebrafish)
    

    Use

    In vitro transcription

    Promoter

    SP6/T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac SOX 318-320A

    Plasmid

    #192060

    Purpose

    Expression vector for SOX (ORF37) 318-320A

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (ORF37)
    

    Expression

    Insect

    Mutation

    318-320A

    Promoter

    Polyhedrin

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid

    #99696

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225062

    Purpose

    Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

    Plasmid

    #225061

    Purpose

    Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225063

    Purpose

    Expression of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac1-NpSxph

    Plasmid

    #193097

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin
    

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac1-NpSxphI559Y

    Plasmid

    #193099

    Purpose

    Protein expression in insect cells

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Nanorana parkeri saxiphilin I559Y
    

    Tags

    3C-eGFP-His10

    Expression

    Insect

    Mutation

    I559Y

    Promoter

    polyhedrin

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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