We narrowed to 19,291 results for: CAL

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pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

Plasmid

#199355

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP-P2A-ALFA-SUMOstar

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

Plasmid

#199365

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA4-CTCF-3p-UTR

Plasmid

#195106

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF 3' UTR region

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA3-CTCF-3p-UTR

Plasmid

#195105

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF 3' UTR region

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-CTCF-prom

Plasmid

#195104

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF promoter

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SuperNovaGreen-T2A-dTomato

Plasmid

#193798

Purpose

Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

SuperNova Green

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mGFP-MutCREB(S133A)

Plasmid

#194642

Purpose

Expresses the fusion of monomeric-EGFP-tagged mutant CREB(S133A) in a Cre-dependent manner

Depositor

Jun-Hyeong Cho

Article

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

CREB (Creb1 Rat)

Use

AAV

Tags

mEGFP

Mutation

S133A

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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TERTpWT_Gluc_CCR5

Plasmid

#192270

Purpose

Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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DDX27-1

Plasmid

#155514

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX27 (DDX27 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-aIIb

Plasmid

#189796

Purpose

Expression of full length human integrin aIIb

Depositor

Timothy Springer

Article

Lin et al Cell. 2022 Sep 15;185(19):3533-3550.e27.

Insert

aIIb (ITGA2B Human)

Expression

Mammalian

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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eDrTrkA

Plasmid

#183996

Purpose

Optically controlled eDrTrkA expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkA (NTRK1 Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179012

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_ss-SBP-EGFP

Plasmid

#172358

Purpose

Reporter to monitoring a streptavidin-binding peptide trafficking

Depositor

Guido Kroemer

Article

Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

Insert

ER importing signal sequence

Use

Lentiviral

Tags

SBP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2-MACROD1

Plasmid

#172587

Purpose

For transient expression of C-terminal mRuby2-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_ZYMND11_coiled-coil MYND

Plasmid

#178493

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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NgΔIQ FUNgDIQGW

Plasmid

#92232

Purpose

Lentiviral expression of human Ng with deletion mutation

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

Ng (NRGN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

delta IQ

Promoter

hUbC

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-SECFP

Plasmid

#175826

Purpose

Express SECFP with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRD406

Plasmid

#163111

Purpose

Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mNeonGreen_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRD398

Plasmid

#163105

Purpose

Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_mCherry_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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