We narrowed to 19,276 results for: Tat
-
Plasmid#145155PurposeMammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 1DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFc region of human IgA1ExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-beta2 RD-1-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128343PurposepAAV encoding gRNA sequence for loss-of-function indelsDepositorAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL681 CHYRON4 integration construct
Plasmid#126449PurposeTo integrate the CHYRON4 locus at AAVS1 in human cells.DepositorInsertspU6/3xLacO-CHYRON4 hgRNA
pCMV-puro
ExpressionMammalianMutationThe SpCas9 sgRNA constant region is mutated to ma…PromoterCMV and human U6/3xLacOAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2
Plasmid#118170PurposeCRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.DepositorInsertKRAB-dCas9-2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhPGK and U6Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE
Plasmid#111387PurposeAAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)DepositorAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKAsub(TA)-TagRFPT-CAAX
Plasmid#87740PurposeNegative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).DepositorInsertPKAsub(TA)-TagRFPT-CAAX
Tags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SOCS6-WT
Plasmid#74496PurposeOverexpression of SOCS6 in mammalian cellsDepositorAvailable SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-L647RLaminA-CS
Plasmid#69062Purposeencodes a lamin A mutant with Leucine 647 mutated to an Arginin and with a substitution of cysteine in CaaX-motif by a serineDepositorInsertLaminA (LMNA Human)
UseRetroviralExpressionMammalianMutationLamin A with Leucine 647 mutated to Arginine and …PromoterCMVAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin E
Plasmid#68819PurposeFlag tagged mammalian expression of E domainDepositorInsertGephyrin (Gphn Rat)
Tags3xFLAGExpressionMammalianMutationinternal EcoRI site has silent mutationPromoterCMVAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-ALG13 (OTU, aa 216-353)
Plasmid#61414PurposeExpresses human ALG13 (OTU domain) in E. coli.DepositorInsertALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-215 and aa 354-1137.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLuc-CDS-m215-2
Plasmid#42102DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsRenilla luciferaseExpressionMammalianMutationSilent mutations were introduced in the seed-comp…PromoterSV40Available SinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P3nmt1-GST
Plasmid#39286DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Mutation-1163 to +6 of nmt1 locusAvailable SinceSept. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA mouse NHERF-1 (1-325)
Plasmid#32706DepositorInsertsolute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)
TagsnoneExpressionMammalianMutationDeleted last 30 AA putative Ezrin binding sitePromoterCMVAvailable SinceFeb. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH371-SUP45-N58A
Plasmid#29378DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationN58A mutant gene including genomic sequence 1432 …Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3b(1454/3172)P2P-mut
Plasmid#21405DepositorInsertEGLN1 (EGLN1 Human)
UseLuciferaseExpressionMammalianMutationhypoxia response element mutatedAvailable SinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
RB-409-815-pGex2T
Plasmid#21784DepositorInsertRb (RB1 Human)
TagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 409-815 of wt Rb …Available SinceAug. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCSP1-mp53AS-6xMUT
Plasmid#20904DepositorInsertp53AS (Trp53 Mouse)
ExpressionMammalianMutationpoint mutation (Ser/Thr-Ala) of the 6 N-terminal …Available SinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG180 SGCD 3'UTR mut
Plasmid#12038DepositorInsertN13 3'UTR mut (SGCD Human)
UseLuciferaseExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…Available SinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only