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POT4-TEF2p-ycrtI-ADH1t

Plasmid

#65356

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtI-ADH1t

Use

Unspecified

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TEF2

Plasmid

#65337

Purpose

construction of TEF2 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 promoter

Use

Synthetic Biology

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFAB822

Plasmid

#47856

Purpose

BIOFAB reporter plasmid for measuring BBa_B1006 termination efficiency

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

BBa_B1006 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO11

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB803

Plasmid

#47847

Purpose

BIOFAB reporter plasmid for measuring T7 early termination efficiency

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T7 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO2

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pUC-BamHI-24xMS2V6-EcoRI

Plasmid

#255008

Purpose

adding homology regions to 24xMS2V6

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

24xMS2V6

Use

Unspecified

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pUC-BamHI-12xMS2V6-EcoRI

Plasmid

#255009

Purpose

adding homology regions to 12xMS2V6

Depositor

Silke Hauf

Publication

Weidemann et al J Cell Sci. 2026 Jul 1;139(13):jcs264809

Insert

12xMS2V6

Use

Unspecified

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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ABE8e-YA

Plasmid

#238085

Purpose

A High-Fidelity Adenine Base Editor with YA Context Preference

Depositor

Xiaohui Zhang

Publication

Shang et al Nat Commun. 2025 Oct 15;16(1):9153. doi:

Insert

TadA-8e (A48E)-nSpCas9

Use

CRISPR

Expression

Mammalian

Available since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pET15b-TGP-TwinStrep

Plasmid

#240251

Purpose

IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

Depositor

Olga Boudker, Krishna Reddy

Publication

Reddy et al Nat Struct Mol Biol (2025).

Type

Empty backbone

Tags

10x HIS, Thrombin, Twin-Strep, and thermostable G…

Expression

Bacterial

Promoter

T7

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK2_Rluc-Y0x-Fluc

Plasmid

#233614

Purpose

Expression of Rluc-Fluc reporter gene containing no Y

Depositor

Shintaro Iwasaki

Publication

Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

Insert

Rluc-Y0x-Fluc

Use

Luciferase

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.minBG-CI-SaCas9-W3SynpA

Plasmid

#231369

Purpose

minBG-driven SaCas9, with short synthetic terminator sequence.

Depositor

Viviana Gradinaru

Publication

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

SaCas9

Use

AAV

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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proinsulin-msGFP2 (C48S C70S)

Plasmid

#218956

Purpose

Expresses proinsulin fused to msGFP2 (C48S C70S) in bacteria

Depositor

Benjamin Glick

Insert

proinsulin-msGFP2 C48S C70S

Tags

proinsulin

Expression

Bacterial

Promoter

T5

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph

Plasmid

#218366

Purpose

Empty backbone for Tn7-site insertion

Depositor

Michael Gebhardt

Publication

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mStrawberry-15b

Plasmid

#206868

Purpose

Express tTALE-mStrawberry

Depositor

Kyubong Jo

Publication

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mStrawberry

Expression

Bacterial

Promoter

T7 promoter

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS021

Plasmid

#207917

Purpose

Level 0 for gRNA in E2 module (5' CCAT-3' ACTA)

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKE013

Plasmid

#207916

Purpose

Level 0 for gRNA in E1 module (5' CTGC-3' CCAT)

Depositor

Alexis Maizel

Publication

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W275L

Plasmid

#196019

Purpose

Expresses CRKL W275L

Depositor

William Hahn

Publication

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pZS220

Plasmid

#177642

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Publication

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K47A

Available since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-HIS3

Plasmid

#122887

Purpose

LYS2-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-MET17

Plasmid

#122889

Purpose

LYS2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-LEU2

Plasmid

#122892

Purpose

TRP1-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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