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pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Plasmid

#92392

Purpose

produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Depositor

Hyungbae Kwon

Article

Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Human Synapsin

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCasKP-apr

Plasmid

#117231

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MAVS-Mito

Plasmid

#44556

Purpose

Retroviral vector for expression of a chimeric MAVS allele targeted to mitochondria in mammalian cells

Depositor

Jonathan Kagan

Article

Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

Insert

MAVS-Fis1 chimeric protein (MAVS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

contains aa1-500 of MAVS and aa127-152 of Fis1

Promoter

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only
pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-N1

Plasmid

#54730

Purpose

Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-PML IV/pRK5

Plasmid

#59742

Purpose

Expresses the isoform IV of Promyelocytic Leukemia Protein (PML) in mammalian cells

Depositor

Xiaolu Yang

Article

Guo et al Mol Cell. 2014 Jul 3;55(1):15-30. doi: 1

Insert

PML isoform IV (PML Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-dCas9-BFP

Plasmid

#46910

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, and HA

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCR3.1-mGM-CSF

Plasmid

#74465

Purpose

expression of mGM-CSF in mammalian cells

Depositor

Loree Heller

Article

Heller et al Melanoma Res. 2000 Dec;10(6):577-83.

Insert

granulocyte-monocyte colony-stimulating factor (Csf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCCL/IFNB1-d2eGFP-3’UTR

Plasmid

#180232

Purpose

Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

Insert

d2eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1 kb upstream of IFNB1 CDS

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PX333-T2A-EGFP

Plasmid

#197417

Purpose

SpCas9-T2A-EGFP with dual sgRNA cloning backbones

Depositor

Yeh-Hsing Lao

Article

Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CBh; U6

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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