162,160 results

1. pcDNA C12 ecDHFR DD YFP HA

   Plasmid

   #192815

   Purpose

   Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.

   Depositor

   John Hulleman

   Article

   Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021

   Insert

   E. coli dihydrofolate reductase
   

   Use

   Tags

   enhanced yellow fluorescent protein and hemagglut…

   Expression

   Mammalian

   Mutation

   W74R/T113S/E120D/Q146L

   Promoter

   CMV

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWS5397-CRISPRai-NatR

   Plasmid

   #182836

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRC/CMV-TYK2-VSV

   Plasmid

   #139344

   Purpose

   Expression of TYK2 protein

   Depositor

   Sandra Pellegrini

   Article

   Li et al PLoS One. 2020 Jan 21;15(1):e0225289. do

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-3FLAG-EEA1

   Plasmid

   #176491

   Purpose

   For lentiviral transduction of 3FLAG-EEA1

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEB2-2ndVal mScarlet-I

   Plasmid

   #104028

   Purpose

   Plasmid encoding 2ndVal mScarlet-I

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   2ndVal mScarlet-I
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   mScarlet-I with valine inserted in 2nd position

   Promoter

   proC

   Available since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF2848

   Plasmid

   #144249

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF21 (TCF21 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRDA_816

   Plasmid

   #201157

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D908A

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ABCA1 promoter luciferase reporter (Full-length)

   Plasmid

   #86442

   Purpose

   Human ABCA1 promoter luciferase reporter (Full-length)

   Depositor

   David Mu

   Article

   Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

   Insert

   ABCA1 promoter (ABCA1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-PIK3CA

   Plasmid

   #23466

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   A1035V

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-hSyn-ConVERGD-(HA)hM3Dq-W3SL

    Plasmid

    #218758

    Purpose

    Provides AND intersectional (Cre+Flp) expression of HA-hM3Dq

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    hM3Dq
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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