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pFRT-DHX36iso1

Plasmid

#159585

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

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ART_RTctrl

Plasmid

#214066

Purpose

Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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EXT1-pLJC2-3XFLAG

Plasmid

#215818

Purpose

Ectopic expression of EXT-1 in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-ultraID

Plasmid

#207682

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1 Lifeact-EGFP-2XNLS

Plasmid

#58467

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Lifeact
2X NLS with 2X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-ZEAXipi

Plasmid

#53287

Purpose

Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

Insert

crtE, idi, crtY, crtI, crtB, crtZ

Use

Low copy number bacterial cloning vector

Tags

Expression

Mutation

Promoter

endogenous promoters

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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CMV500 A-CREB

Plasmid

#33371

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

CREB (Creb1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available since

Dec. 19, 2011

Availability

Academic Institutions and Nonprofits only

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Venus-PTP1b

Plasmid

#234740

Purpose

Mammalian expression of endoplasmic reticulum marker Venus-PTPb

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-PTP1b

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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mRuby2-PTP1b

Plasmid

#234748

Purpose

Mammalian expression of endoplasmic reticulum marker mRuby2-PTPb

Depositor

Nevin Lambert

Article

Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

Insert

mRuby2-PTP1b

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha-pcDNA3

Plasmid

#18949

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

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