166,289 results

1. pH2B-Electra2

   Plasmid

   #179479

   Purpose

   Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   H2B-Electra2
   

   Tags

   Electra2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pISH-Dlx5

   Plasmid

   #74344

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx5. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   distal-less homeobox 5 (Dlx5 Mouse)
   

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-NQL007-NANOG-sgRNA

   Plasmid

   #175555

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII

   Plasmid

   #80863

   Purpose

   Phagemid (encodes evolved cI4A5T6T,P and M13 gene III)

   Depositor

   Mark Isalan

   Article

   Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

   Inserts

   M13 gene III
   cI4A5T6T,P
   

   Expression

   Bacterial

   Promoter

   BBa_J23106 and rpoN

   Available Since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSB-hsa-miR-99b-5p

   Plasmid

   #103767

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-99b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-99b-5p target (MIR99B Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ2214 - Intron GG1 - 900 bp

   Plasmid

   #159880

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   Intron GG1 - 900 bp
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LSB-hsa-miR-25-3p

   Plasmid

   #103375

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-25-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-25-3p target (MIR25 Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pISH-Dlx6

   Plasmid

   #74345

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx6. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   distal-less homeobox 6 (Dlx6 Mouse)
   

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. LSB-hsa-miR-30a-5p

   Plasmid

   #103410

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-30a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-30a-5p target (MIR30A Human)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN_TmiR_G13-L_G12-E

    Plasmid

    #25763

    Purpose

    Entry vector with TRE promoter driving both mouse G alpha 12 and G alpha 13 miR30-based shRNAs.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 and 12 miR-shRNA
    

    Use

    Entry vector

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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