We narrowed to 22,441 results for: scr

1. pLenti-sgRNA-Lib

   Plasmid

   #53121

   Purpose

   sgRNA expression construction in a 3rd generation lentiviral backbone

   Depositor

   Wensheng Wei

   Article

   Zhou et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-mCherry

   Plasmid

   #66534

   Purpose

   Expresses mCherry under the control of the Concensus T7 Promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon Optimized for E. coli

   Promoter

   Consensus T7

   Available since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-SNAP-TRIM21

   Plasmid

   #196181

   Purpose

   For in vitro transcription of SNAP-TRIM21 mRNA

   Depositor

   Binyam Mogessie

   Article

   Dunkley et al Sci Adv. 2023 Jan 20;9(3):eadc9161. doi:

   Insert

   SNAP-TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXPR_124

   Plasmid

   #133461

   Purpose

   EF1a promoter expresses SpyoCas9 and P2A provides EGFP

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   SpyoCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiex-1 Myosin VI HMM_eGFP

   Plasmid

   #82714

   Purpose

   Expresses Myosin VI HMM with a C-terminal eGFP.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Myosin VI
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC90-pmax-DEST

   Plasmid

   #48222

   Purpose

   Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   CRISPR; Gateway destination

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. BirA-Myc_N_SP1

   Plasmid

   #167726

   Purpose

   Expresses SP1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   SP1 (SP1 Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cmv d0 dPspCas13b-GGS-NYTHDF1

   Plasmid

   #119855

   Purpose

   Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

   Depositor

   Bryan Dickinson

   Article

   Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

   Insert

   dCas13b-GGS-NYTHDF1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AL13Rb-35S

   Plasmid

   #161008

   Purpose

   PP7-tagged reporter driven by the 35S promoter

   Depositor

   Krishna Niyogi

   Article

   Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

   Insert

   PP7 and H2B-mScarlet
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pxpr_502-wt-ltr

    Plasmid

    #182647

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_449

    Plasmid

    #179093

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3 GATA Luc

    Plasmid

    #85695

    Purpose

    Luciferase reporter containing three repeats of the GATA consensus site

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    3xGATA and minimal MT promoter
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    3xGATA and minimal MT promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

13. p4RTK GL4.10 (Luc2)

    Plasmid

    #16057

    Depositor

    Robert Benezra

    Article

    Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

    Insert

    Multimerized E-box
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. PKC beta II CAT

    Plasmid

    #16384

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC beta II (Prkcb Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CAT: catalytic domain, aa 329-673

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ154

    Plasmid

    #69305

    Purpose

    Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9 (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP NFAT5 DBD

    Plasmid

    #14112

    Depositor

    Anjana Rao

    Article

    Jauliac et al Nat Cell Biol. 2002 Jul . 4(7):540-4.

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    DNA binding domain aa 175 to 471

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

17. FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1

    Plasmid

    #58432

    Purpose

    Induces Citrine expression from a promoter containing 1 lexA box in yeast cells

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    CitrineA206K
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    insul-(lexA-box)1-PminCYC1

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAD6

    Plasmid

    #134349

    Purpose

    E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

    Depositor

    Seth Darst

    Article

    Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    mfd (mfd E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-mKate2-NS

    Plasmid

    #109729

    Purpose

    Multisite gateway vector for 5' tagging with mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    mKate2
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Use

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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