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pHR-SFFV-FLAG-TurboID-mutLIR-SQSTM1

Plasmid

#223718

Purpose

Expresses FLAG-TurboID-mutLIR-SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

Mutation 335-DDDW-338 to 335-AAAA-338

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pESC-LEU-HsAIDSc

Plasmid

#60810

Purpose

to express human AID recoded for yeast codon usage

Depositor

Thomas Kunkel, Youri Pavlov

Article

Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

Insert

AID (AICDA Human)

Tags

Myc

Expression

Yeast

Mutation

codon optimized for yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pFRT/TO/HIS/FLAG/HA-MYEF2

Plasmid

#38069

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

MYEF2 myelin expression factor 2 (MYEF2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGEM-HSPA5P

Plasmid

#44150

Purpose

a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

Depositor

Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

HSPA5 promoter (HSPA5 Human)

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-dLight1.2

Plasmid

#111069

Purpose

To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV1

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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LSB-hsa-miR-195-3p

Plasmid

#103311

Purpose

Used to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

Depositor

Ron Weiss

Article

Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

Insert

hsa-miR-195-3p target (MIR195 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pIE-hα2M-H6

Plasmid

#128492

Purpose

Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (A2M Human)

Tags

His tag (6x)

Expression

Insect

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDest27-mBIN1+13

Plasmid

#61087

Purpose

Express N-terminal GST tagged-mouse BIN1+13 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

mouse BIN1 with exon 13, excluding exon 7, 11, 14-17

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pDest27-mBIN1

Plasmid

#61086

Purpose

Express N-terminal GST tagged-mouse BIN1 in mammalian cells

Depositor

Robin Shaw

Article

Hong et al Nat Med. 2014 Jun;20(6):624-32. doi: 10.

Insert

smallest constitutive BIN1excluding exon 7, 11, 13-17

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pET-pro-siRNA-V2-TP53

Plasmid

#111089

Purpose

pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human TP53 gene.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Insert

TP53 (TP53 Human)

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-q-pep

Plasmid

#47437

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pDsred monomer C1- MLPH

Plasmid

#89236

Purpose

Expression of Dsred-tagged human Melanophilin in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

Human Melanophilin (MLPH Human)

Tags

Dsred monomer

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5-FLAG-Gene33

Plasmid

#21633

Depositor

John Kyriakis

Article

Makkinje et al J Biol Chem. 2000 Jun 9. 275(23):17838-4

Insert

Gene33 (Errfi1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-K660E

Plasmid

#45705

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660E

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Crk-SH2

Plasmid

#46418

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Crk (CRK Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-124

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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shFGF2#2

Plasmid

#157668

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSI-535

Plasmid

#131130

Purpose

gRNA expression vector for C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

C-to-T reporter activation gRNA

Use

CRISPR

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

Plasmid

#187959

Purpose

Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Inserts

Blasticidin resistance
FKBP12_F36V

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAS18

Plasmid

#158787

Purpose

Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Ptag-168::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pQCascade-IS186

Plasmid

#140627

Purpose

Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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