We narrowed to 10,965 results for: T7
-
Plasmid#205970PurposeMWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)DepositorInsertCas5-HNH all proteins, with His-tagged Cas11
Tags14xHis on Cas11ExpressionBacterialPromoterT7Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_dHMGA_pET28
Plasmid#167329PurposeExpresses TFAM dHMGA mutant in bacterial cellsDepositorInsertTFAM (TFAM Human)
Tags6XHisExpressionBacterialMutationTruncation mutant containing linker, HMGB, and C-…PromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-StefinA
Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
TagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
KCC2B_HUMAN_D0
Plasmid#79718PurposeThis plasmid encodes the kinase domain of KCC2B. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKCC2B
TagsHis10-TEVExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HCF-1rep1E10A
Plasmid#84607PurposePlasmid contains HCF-1rep1E10A with GST-tagDepositorAvailable SinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HCF-1rep1wt
Plasmid#84606PurposePlasmid contains HCF-1rep1wt with GST-tagDepositorAvailable SinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXA4 (murine) HA-tag pET
Plasmid#8489DepositorInsertHOXA4 (Hoxa4 Mouse)
TagsHA and T7ExpressionBacterialMutationThr-2 changed to Ala-2 to form better ribosomal b…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXB2 (human) HIS-tag pET
Plasmid#8522DepositorInsertHOXB2 (HOXB2 Human)
TagsHis and T7ExpressionBacterialMutationStarts at AA #2; makes protein by WesternAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FAM86A
Plasmid#85117Purposebacterial expression of FAM86ADepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub(TA)-YPet-NES
Plasmid#84636PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub(TA)-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
VGFR1_HUMAN_D0
Plasmid#79743PurposeThis plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVGFR1
TagsHis10-TEVExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBQLN2_450-624W
Plasmid#176852PurposeExpresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cellsDepositorInsertUBQLN2_450C_W (UBQLN2 Human)
ExpressionBacterialMutationdeletion of residues 1-449 and addition of C-term…PromoterT7Available SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Rhodoplanes-bGSDM
Plasmid#212129PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRhodoplanes bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM
Plasmid#212127PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertVitiosangium bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Gillisia-bGSDM
Plasmid#212128PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertGillisia bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bradyrhizobium-bGSDM
Plasmid#212126PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBradyrhizobium bGSDM
Tags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available SinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-GFP(S45C)-ZdK1
Plasmid#185791PurposeEncodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linkerDepositorInsertRBP-sfGFP(S45C)-ZdK1
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationSer to Cys mutation at start of GFP, residue 45 i…PromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBeast-pT7-soxA
Plasmid#190057PurposeExpress the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7DepositorInsertSoxA
UseSynthetic Biology; Cell-free protein synthesisAvailable SinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_KPNA4_Arm
Plasmid#178495PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only