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pCDNA-gp78-FLAG RING mutant C356G H361A

Plasmid

#61751

Purpose

express C-terminally FLAG tagged human gp78

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

gp78 (AMFR Human)

Tags

FLAG

Expression

Mammalian

Mutation

C356G H361A; P179L, F262L, Y315C, and D346G (ple…

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pGWB516

Plasmid

#74858

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB550

Plasmid

#74880

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-nArgBP2

Plasmid

#74514

Purpose

Express GFP-nArgBP2 fusion protein in mammalian cells

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

nArgBP2 (Sorbs2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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AXL gRNA (BRDN0001146797)

Plasmid

#76700

Purpose

3rd generation lentiviral gRNA plasmid targeting human AXL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AXL (AXL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-PGBD5

Plasmid

#78121

Purpose

Lentiviral gene expression vector for doxycycline inducible PGBD5 expression

Depositor

Alex Kentsis

Article

Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

Insert

PGBD5 (PGBD5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pFM699

Plasmid

#74636

Purpose

alias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

mCherry

Expression

Yeast

Promoter

none

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-Mff(1-61)-PP-GST

Plasmid

#73042

Purpose

Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

Depositor

David Chan

Article

Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

Insert

Mff(1-61) (Mff Mouse)

Tags

GST-tag and PreScission protease site

Expression

Bacterial

Mutation

Truncation containing amino acids 1-61

Promoter

T7

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pGM202T7

Plasmid

#69993

Purpose

E. coli/Streptomyces expression vector containing the T7-promoter

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aphII
tsr
rep
sso
T7 promoter
dso
His-Orf

Tags

N-terminal and C-terminal His tag encoding sequen…

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pBrain-ch-TOGKDP-GFP-shch-TOG

Plasmid

#69113

Purpose

Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

Depositor

Stephen Royle

Article

Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

Inserts

Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
shch-TOG

Use

RNAi

Tags

EGFP

Expression

Mammalian

Mutation

Silent mutations to confer shRNA resistance.

Promoter

CMV

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4 K41M-3xFLAG

Plasmid

#69838

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF6-GFP

Plasmid

#67394

Purpose

Mammalian expression of hArf6 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL3-GFP

Plasmid

#67397

Purpose

Mammalian expression of hArl3 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
Cav3-YFP

Plasmid

#69507

Purpose

Expression of fluorescently tagged Caveolin-3 in mammalian cells

Depositor

Rob Parton

Article

Pol et al Mol Biol Cell. 2004 Jan;15(1):99-110. Ep

Insert

Cav3 (Cav3 Mouse)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pmPB2-VSV

Plasmid

#68038

Purpose

Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Tags

VSV

Expression

Mammalian

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Poly(A)‐NeoR

Plasmid

#65526

Purpose

NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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attB-GFP

Plasmid

#65521

Purpose

Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Slc17a7-IRES2-Cre targeting vector

Plasmid

#61574

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Slc17a7-IRES2-Cre

Use

Cre/Lox and Mouse Targeting

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pFPL-Rg

Plasmid

#61654

Purpose

Gateway vector for generating red fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Expression

Plant

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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