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Plasmid#108813PurposeExpresses N-terminus to aa326 of TP901 integrase fused to GID1 CIDDepositorInsertTP901 integrase fragment N-terminus to aa326
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpA
Plasmid#108801PurposeExpresses N-terminus to aa428 of PhiC31 integrase fused to ABI CIDDepositorInsertPhiC31 integrase fragment N-terminus to aa428
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2278_pCAG-PV1-FlpO-N168-L1-ABI-NLS-BGHpA
Plasmid#108765PurposeExpresses N-terminus to aa168 of Flp recombinase fused to ABI CIDDepositorInsertFlp recombinase fragment N-terminus to aa168
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpA
Plasmid#108766PurposeExpresses aa169 to C-terminus of Flp recombinase fused to PYL CIDDepositorInsertFlp recombinase fragment aa169 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA
Plasmid#108727PurposeExpresses N-terminus to aa256 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa256
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2231_pCAG-PV1-GAI-L1-iCre-257C-NLS-BGHpA
Plasmid#108728PurposeExpresses aa257 to C-terminus of Cre recombinase fused to GAI CIDDepositorInsertCre recombinase fragment aa257 to C-terminus
UseCre/Lox and Synthetic BiologyTagsGAI and NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJM582 EF1α 3xFLAG-NLS-VP64-ZF2 in TUPV2
Plasmid#161543PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF2 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF2
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2
Plasmid#161533PurposeConstitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-ZF1
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101111PurposeEncodes dCas9(C)-VP64 with SAM components fused to AVPR2DepositorInsertAVPR2-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
Plasmid#159800PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW2689_pCAG-PV1-iCre-N229-L1-nMag-NLS-BGHpA
Plasmid#108817PurposeExpresses N-terminus to aa229 of Cre recombinase fused to nMag CIDDepositorInsertCre recombinase fragment N-terminus to aa229
UseCre/Lox and Synthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2210_pCAG-PV1-iCre-N251-L1-GID1-NLS-BGHpA
Plasmid#108725PurposeExpresses N-terminus to aa251 of Cre recombinase fused to GID1 CIDDepositorInsertCre recombinase fragment N-terminus to aa251
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64
Plasmid#101110PurposeEncodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2DepositorInsertBDKRB2-dCas9(C)_DHFR-PCP-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA
Plasmid#108844PurposeInsert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA
Plasmid#108815PurposeExpresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CIDDepositorInsertBxb1 integrase fragment N-terminus to aa468
UseCre/Lox and Synthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2678_pCAG-PV1-pMag-L1-FlpO-397C-NLS-BGHpA
Plasmid#108832PurposeExpresses aa397 to C-terminus of Flp recombinase fused to pMag CIDDepositorInsertFlp recombinase fragment aa397 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and pMagExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpA
Plasmid#108785PurposeExpresses N-terminus to aa269 of VCre recombinase fused to ABI CIDDepositorInsertVCre recombinase fragment N-terminus to aa269
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpA
Plasmid#108786PurposeExpresses aa270 to C-terminus of VCre recombinase fused to PYL CIDDepositorInsertVCre recombinase fragment aa270 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianMutationPromoterpCAGAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmyc-GFP-TNRC6A-(925-934)-NLS-mut
Plasmid#42020DepositorInsertTNRC6A (TNRC6A Human)
UseTagsEGFP and MycExpressionMammalianMutationcontain aa925-934, R930A, R931A, R932A, R934APromoterCMVAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP
Plasmid#226114PurposeMammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.DepositorInsertAcrIIC3
UseTagsFLAG and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)
Plasmid#229811PurposeA Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)DepositorInsertLexA transactivator-LexA-Operon-nls-GFP
UseTol2 based expression vectorTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP WPRE bGH pA (JDW 488)
Plasmid#224540PurposeA Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleusDepositorInsertnls-EGFP-WPRE-bGH-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
Plasmid#217970PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.DepositorInsert2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4
Plasmid#219711PurposeLevel 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPnls barcode-210-SV40 polyA
Plasmid#190876PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode210
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK mCh-V5-NES-EGFP-EGFR NLS zeo
Plasmid#192337PurposeLentiviral expression vector for an inducible mCh-V5-NES-EGFP-EGFR NLSDepositorInsertmCh-V5-NES-EGFP-EGFR NLS (EGFR Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo
Plasmid#192339PurposeLentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLSDepositorInsertmCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK mCh-V5-NES-EGFP-SV40 NLS zeo
Plasmid#192340PurposeLentiviral expression vector for an inducible mCh-V5-NES-EGFP-SV40 NLSDepositorInsertmCh-V5-NES-EGFP-SV40 NLS
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro
Plasmid#173232PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1333N)-UGI-Puro
Plasmid#173233PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1397C)-UGI-Puro
Plasmid#173234PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NLS-ccdb-DddA(G1397N)-UGI-Puro
Plasmid#173235PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)
Plasmid#179080PurposeParent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminatorsDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-VP64-MCS_N-term
Plasmid#136886PurposeDoxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertVP64 activator domain
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-KRAB-MCS_N-term
Plasmid#136888PurposeDoxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertKRAB repressor domain
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM554 EF1α 3xFLAG-NLS-VP64-intN in TUPV2
Plasmid#161556PurposeConstitutive expression of 3xFLAG-NLS-VP64-intN under the EF1α promoterDepositorInsert3xFLAG-NLS-VP64-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM555 EF1α 3xFLAG-NLS-DsDed-intN in TUPV2
Plasmid#161557PurposeConstitutive expression of 3xFLAG-NLS-DsDed-intN under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM571 ZF2x6-C 3xFLAG-NLS-VP64-intN in TUPV2
Plasmid#161558PurposeInducible expression of 3xFLAG-NLS-VP64-intN under ZF2x6-C the promoterDepositorInsert3xFLAG-NLS-VP64-intN
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterZF2x6-CAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only