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We narrowed to 6,542 results for: poly

Showing: 2101 - 2150 of 6542 results

  1. pOpen-9N7polA

    Plasmid
    #165500
    Purpose
    Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    9 degrees N-7 DNA polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165500-plasmid-map-sequence-id-338428

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  2. pOpen-Bstpol

    Plasmid
    #165543
    Purpose
    Full length DNA polymerase from Bacillus stearothermophilus.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Bst DNA Polymerase, Full Length
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165543-plasmid-map-sequence-id-339279

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  3. pOpen-TthpolA

    Plasmid
    #165533
    Purpose
    DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Tth DNA polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165533-plasmid-map-sequence-id-339122

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  4. pBS-2xpA

    Plasmid
    #176504
    Purpose
    2x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    2x SV40 polyA
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176504-plasmid-map-sequence-id-348023

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  5. pBS-4xpA

    Plasmid
    #176506
    Purpose
    4x SV40 polyA sequences
    Depositor
    Mario Capecchi
    Insert
    4x SV40 polyA
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176506-plasmid-map-sequence-id-347136

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  6. pOpen-SP6rpol

    Plasmid
    #165516
    Purpose
    DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    SP6 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165516-plasmid-map-sequence-id-338647

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  7. pCAG-VSVL

    Plasmid
    #64085
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain polymerase gene
    Depositor
    Ian Wickersham
    Insert
    VSV polymerase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64085-plasmid-map-sequence-id-429028

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  8. pOpen-9N7polA (CT)

    Plasmid
    #165502
    Purpose
    9 Degrees North DNA polymerase variant with enhanced ability to incorporate modified substrates
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    9 degrees N-7 DNA Polymerase chain terminating
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165502-plasmid-map-sequence-id-338438

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  9. pJY4092

    Plasmid
    #113334
    Purpose
    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Insert
    codon-optimized Hermes transposase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113334-plasmid-map-sequence-id-218285

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  10. C95m

    Plasmid
    #121001
    Purpose
    MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7 RNA polymerase (Andy please check!)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121001-plasmid-map-sequence-id-236130

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  11. 371-A

    Plasmid
    #97026
    Purpose
    yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97026-plasmid-map-sequence-id-182505

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  12. pOpen-GBDpol

    Plasmid
    #165501
    Purpose
    Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Pyrococcus Sp. Heat-Stable DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 26, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165501-plasmid-map-sequence-id-338429

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  13. pOpen-K12polLF

    Plasmid
    #165511
    Purpose
    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    DNA Polymerase I, Large (Klenow) Fragment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165511-plasmid-map-sequence-id-338596

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  14. pOpen-T5gene122

    Plasmid
    #165544
    Purpose
    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T5 DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165544-plasmid-map-sequence-id-339280

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  15. 371-U

    Plasmid
    #97036
    Purpose
    MYFQSNA-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    MYFQSNA
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97036-plasmid-map-sequence-id-182502

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  17. pCAG-B19L

    Plasmid
    #59922
    Purpose
    Expression vector for Rabies virus SAD B19 RNA dependent RNA polymerase gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus RNA dependent RNA polymerase
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    59922-plasmid-map-sequence-id-94980

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  18. pOpen-THEAQpolA

    Plasmid
    #165512
    Purpose
    DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Taq DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165512-plasmid-map-sequence-id-339400

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  19. pOpen-T3gene1

    Plasmid
    #165576
    Purpose
    DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    T3 RNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165576-plasmid-map-sequence-id-340988

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  20. pOpen-dbh

    Plasmid
    #165513
    Purpose
    Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    Sulfolobus DNA Polymerase IV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165513-plasmid-map-sequence-id-338598

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  21. pMCMV3

    Plasmid
    #85711
    Purpose
    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences
    Depositor
    Ulrich Koszinowski, Martin Messerle
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MIEPmouse CMV MIEP
    Available since
    April 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    85711-plasmid-map-sequence-id-177907

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  22. 371-Bc

    Plasmid
    #97028
    Purpose
    yORF-tev-His6
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97028-plasmid-map-sequence-id-182499

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  23. 371-B

    Plasmid
    #97027
    Purpose
    His6-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97027-plasmid-map-sequence-id-182504

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  24. pSG7163 pLIBT7_SNAPtag-3C-8His

    Plasmid
    #232622
    Purpose
    Expression vector for SNAPtag (carboxy terminal 3C-8His tag)
    Depositor
    Stephan Gruber
    Insert
    SNAPtag-3C-8His
    Use
    Tags
    3C-8His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232622-plasmid-map-sequence-id-468942

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  26. 371-R

    Plasmid
    #97033
    Purpose
    StrepII-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    StrepII tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97033-plasmid-map-sequence-id-182501

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  27. 371-L

    Plasmid
    #97031
    Purpose
    FLAG-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    FLAG tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97031-plasmid-map-sequence-id-182492

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  29. pCAG-N2cL

    Plasmid
    #100812
    Purpose
    express rabies virus CVS N2c strain polymerase gene (N2cL)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain polymerase gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100812-plasmid-map-sequence-id-189505

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  30. TDP43-NTD

    Plasmid
    #241024
    Purpose
    Bacterial expression of TDP-43 NTD
    Depositor
    Nicolas Fawzi
    Insert
    TDP43-NTD
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    changed N81 to stop codon
    Promoter
    Available since
    July 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    241024-plasmid-map-sequence-id-479758

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  31. PKA1248

    Plasmid
    #239369
    Purpose
    S.cerevisiae Sla1-SH3#1-3 (5-413) in pGEX6P1
    Depositor
    Kathryn Ayscough
    Insert
    SLA1 (amino acids 5-413)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239369-plasmid-map-sequence-id-478757

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  32. PKA1247

    Plasmid
    #239368
    Purpose
    S.cerevisiae Sla1-SH3#1-2 (5-131) in pGEX6P1
    Depositor
    Kathryn Ayscough
    Insert
    SLA1 (5-131)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239368-plasmid-map-sequence-id-478747

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  33. pSG7084 pG46-46

    Plasmid
    #232621
    Purpose
    pG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).
    Depositor
    Stephan Gruber
    Type
    Empty backbone
    Use
    Precursor plasmid to generate modified dna circles
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    232621-plasmid-map-sequence-id-468938

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  36. 371-T

    Plasmid
    #97035
    Purpose
    His6-Thioredoxin-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, TEV cleavage site, and Thioredoxin funs…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97035-plasmid-map-sequence-id-182503

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  37. 371-S

    Plasmid
    #97034
    Purpose
    His6-Sumo-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, SUMO domain, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97034-plasmid-map-sequence-id-182498

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  38. 371-O

    Plasmid
    #97032
    Purpose
    His6-Mocr-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, TEV cleavage site, and mOCR fusion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97032-plasmid-map-sequence-id-182497

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  39. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Use
    Tags
    NLS
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47917-plasmid-map-sequence-id-68604

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  41. 371-GFP

    Plasmid
    #97030
    Purpose
    His6-GFP-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    GFP fusion, His6 tag, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97030-plasmid-map-sequence-id-182506

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  44. PKA1317

    Plasmid
    #239371
    Purpose
    S.cerevisiae Sla1-SH3#1-2 (5-131) W108A (mutation preventing binding to 2nd SH3)
    Depositor
    Kathryn Ayscough
    Insert
    SLA1 (5-131)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    W108A
    Promoter
    Available since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239371-plasmid-map-sequence-id-478756

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  45. PKA1279

    Plasmid
    #239375
    Purpose
    GST-Las17 (300-422) P388A
    Depositor
    Kathryn Ayscough
    Insert
    Las17 (amino acids 300-422)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Proline 388 to Alanine
    Promoter
    Available since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239375-plasmid-map-sequence-id-478751

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  46. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97029-plasmid-map-sequence-id-182500

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  50. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

    Plasmid
    #203349
    Purpose
    Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    mGreenLantern
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203349-plasmid-map-sequence-id-399147

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Showing: 2101 - 2150 of 6542 results