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Showing: 841 - 860 of 40900 results

  4. pAAV-TNT4-Zfr-P2A-HA-luciferase-3xMIR122

    Plasmid
    #223184
    Purpose
    Plasmid containing the Zfr/Firefly luciferase/3xMIR122 cassette. Translation of luciferase is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.
    Depositor
    Yuxuan Guo
    Article
    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10
    Inserts
    luciferase (LOC116160065 firefly)
    mir122 (Mir122 Mouse)
    Use
    AAV
    Tags
    HA and engineered P2A
    Promoter
    TNNT2
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    223184-plasmid-map-sequence-id-443757

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  5. pLV-SFFV-RTN3-WPRE-UbC-Emerald

    Plasmid
    #233467
    Purpose
    Lentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    RTN3 (RTN3 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    233467-plasmid-map-sequence-id-465704

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  6. pLV-SFFV-PIK3CA-WPRE-UbC-Emerald

    Plasmid
    #203797
    Purpose
    Lentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Article
    Nieuwenhuis et al EMBO Mol Med. 2020 Aug 7;12(8):e11674. d
    Inserts
    PIK3CA (PIK3CA Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203797-plasmid-map-sequence-id-401572

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  7. STARR-seq luciferase INF enhancer vector_ORI_MX1

    Plasmid
    #99323
    Purpose
    Luciferase validation vector with MX1 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr21: 42791443 -42793515
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    99323-plasmid-map-sequence-id-193152

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  9. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid
    #202571
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202571-plasmid-map-sequence-id-429226

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  10. LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)

    Plasmid
    #132926
    Purpose
    pMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assembly
    Depositor
    Christopher Newgard
    Insert
    Luc2P
    Use
    Luciferase and Synthetic Biology; Pmvp gateway en…
    Tags
    PEST destabilization domain
    Available Since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    132926-plasmid-map-sequence-id-314396

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  11. STARR-seq luciferase INF enhancer vector_ORI_IFIT2

    Plasmid
    #99321
    Purpose
    Luciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr10: 91060605-91062447
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99321-plasmid-map-sequence-id-190859

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  12. pLV-SFFV-CKAP4-WPRE-UbC-Emerald

    Plasmid
    #225943
    Purpose
    Lentiviral vector plasmid expressing human cytoskeleton associated protein 4 (CKAP4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225943-plasmid-map-sequence-id-446819

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  13. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid
    #68218
    Purpose
    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Luc / Renilla / P19
    Use
    Luciferase and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos / 35S / 35S
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68218-plasmid-map-sequence-id-135582

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  15. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid
    #208969
    Purpose
    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    EcKlenow-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208969-plasmid-map-sequence-id-415816

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  16. pLV-SFFV-Emerald-WPRE-UbC-mCherry

    Plasmid
    #211802
    Purpose
    Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    Emerald
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211802-plasmid-map-sequence-id-418109

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  18. pLV-SFFV-RAB10-WPRE-UbC-Emerald

    Plasmid
    #203804
    Purpose
    Lentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    RAB10 (RAB10 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203804-plasmid-map-sequence-id-401585

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  19. RNA polymerase beta prime subunit FLAG

    Plasmid
    #102337
    Purpose
    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.
    Depositor
    Erin O'Shea
    Article
    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1
    Insert
    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    Tags
    3x FLAG with 3X GSGS linker
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102337-plasmid-map-sequence-id-197499

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  20. STARR-seq luciferase INF enhancer vector_ORI_IFI27

    Plasmid
    #99320
    Purpose
    Luciferase validation vector with IFI27 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr14: 94575894-94578286
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    99320-plasmid-map-sequence-id-193160

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Showing: 841 - 860 of 40900 results