We narrowed to 3,108 results for: N-EGFP

1. pEGFP-C1-NuMA

   Plasmid

   #81029

   Purpose

   Expresses human NuMA with N-terminal EGFP tag in mammalian cells

   Depositor

   Michael Mancini

   Insert

   Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65252

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xFLAG-dCas9/MSCV-EGFP

   Plasmid

   #82613

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

   Insert

   3xFLAG-dCas9
   

   Use

   Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-IP-EGFP-ULK1(K46N)

   Plasmid

   #38197

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed Lysine 46 to Asparagine

   Promoter

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

5. Venus-7aa-ActA-pEGFP-C1

   Plasmid

   #177405

   Purpose

   To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

   Depositor

   David Andrews

   Article

   Osterlund et al Protocol Exchange (2022)

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTTH+eGFP-bromo5(N140A)

   Plasmid

   #157806

   Purpose

   Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   eGFP-bromo5(N140A)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   N140A

   Promoter

   pTAC

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-IP-EGFP-ULK2(K39T)

   Plasmid

   #38206

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   Unc-51 like kinase 2 (Ulk2 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed Lysine 39 to Threonine

   Promoter

   Available Since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pMXs-IP-EGFP-ULK1(1-828)

   Plasmid

   #38198

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 829-1051

   Promoter

   Available Since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pT7-EGFP-C1-HsDCP2_1-395_V

   Plasmid

   #147808

   Purpose

   Mammalian Expression of HsDCP2_1-395

   Depositor

   Elisa Izaurralde

   Article

   Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

   Insert

   HsDCP2_1-395 (DCP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nano-lantern-Actin/pEGFP-C1

    Plasmid

    #51972

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pGTag NGFR EGFP nLAP -2

    Plasmid

    #194507

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    NGFR-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-EGFP-DmNot2_402-436_R

    Plasmid

    #147420

    Purpose

    Insect Expression of DmNot2_402-436

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot2_402-436 (Rga Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-EGFP-DmNot2_436-585_R

    Plasmid

    #147421

    Purpose

    Insect Expression of DmNot2_436-585

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot2_436-585 (Rga Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-EGFP-DmNot2_453-585_R

    Plasmid

    #147422

    Purpose

    Insect Expression of DmNot2_453-585

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot2_453-585 (Rga Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-EGFP-DmNot2_469-585_R

    Plasmid

    #147423

    Purpose

    Insect Expression of DmNot2_469-585

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot2_469-585 (Rga Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-EGFP-DmNot2_478-585_R

    Plasmid

    #147424

    Purpose

    Insect Expression of DmNot2_478-585

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot2_478-585 (Rga Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmNot3_677-844_I

    Plasmid

    #146611

    Purpose

    Insect Expression of DmNot3_677-844

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot3_677-844 (Not3 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmNot2_402-585_I

    Plasmid

    #146608

    Purpose

    Insect Expression of DmNot2_402-585

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot2_402-585 (Rga Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGTag NGFR EGFP cLAP +2

    Plasmid

    #194510

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLAP(EGFP)-P2A-NGFR
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGTag NGFR EGFP nLAP +2

    Plasmid

    #194316

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    NGFR-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge