We narrowed to 1,241 results for: post

1. pLVX_TRE3G_DD::mCherry

   Plasmid

   #108679

   Purpose

   Tunable system for controlled gene expression and protein stability in mammalian cells

   Depositor

   Lucia Marucci

   Article

   Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

   Insert

   Destabilizing Domain (DD) mCherry fusion protein
   

   Use

   Lentiviral

   Tags

   DD::mCherry fusion protein

   Expression

   Mammalian

   Mutation

   Promoter

   Doxycycline inducible

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

   Plasmid

   #85070

   Purpose

   Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   PH-PLCD1 (PLCD1 Human)
   

   Use

   Tags

   IRES-sYFP2-PH and mScarlet-H

   Expression

   Mammalian

   Mutation

   PLCD aa 1–170

   Promoter

   CMV

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-C1-TGAT

   Plasmid

   #84334

   Purpose

   Expresses TGAT tagged with mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   TGAT (TRIO Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Dec. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PWPXLD-GFP-Numb-WT

   Plasmid

   #133751

   Purpose

   Wild-type Numb tagged with GFP cloned into PWPXLD

   Depositor

   Chay Kuo

   Article

   Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

   Insert

   GFP-Numb (Numb Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

   Plasmid

   #85069

   Purpose

   Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   PH-PLCD1 (PLCD1 Human)
   

   Use

   Tags

   IRES-sYFP2-PH and mScarlet

   Expression

   Mammalian

   Mutation

   PLCD aa 1–170

   Promoter

   CMV

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-tdTomato-WPRE

   Plasmid

   #133786

   Purpose

   Cre-dependent tdTomato fluorescent reporter for cell labeling

   Depositor

   Michael Stryker

   Article

   Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

   Insert

   pAAV-EF1a-DIO-tdTomato-WPRE
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-Flex-rev-oChIEF-tdTomato

   Plasmid

   #30541

   Purpose

   Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

   Depositor

   Rory McQuiston

   Article

   Bell et al Neuropharmacology. 2011 Aug 25.

   Insert

   oChIEF-tdtomato
   

   Use

   AAV and Cre/Lox

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPC1-GluR1Ctail

   Plasmid

   #32441

   Depositor

   Robert Malinow

   Article

   Rumpel et al Science. 2005 Apr 1;308(5718):83-8. Epub

   Insert

   GluR1 (GRIA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains the last C-terminal 81 amino acids of th…

   Promoter

   CMV

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIVT/Smarca4

   Plasmid

   #122146

   Purpose

   For making Smarca4 RNA

   Depositor

   Carmen Williams

   Article

   Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

   Insert

   Smarca4 (Smarca4 Mouse)
   

   Use

   For in vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWaldo-GFPe_PepTSo

    Plasmid

    #58334

    Purpose

    Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    Simon Newstead, So Iwata

    Article

    Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

    Insert

    PepTso
    

    Use

    Tags

    GFP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pF208wt-luc

    Plasmid

    #122143

    Purpose

    For luciferase expression

    Depositor

    Carmen Williams

    Article

    Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

    Insert

    Fam208a promoter (Tasor Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Fam208a

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pISceI Dusp6:memGFP

    Plasmid

    #32668

    Purpose

    Expresses membrane localized EGFP under the Dusp6 promoter

    Depositor

    Michael Tsang

    Article

    Wang et al Development. 2011 Jan;138(1):45-54. Epub

    Insert

    Dusp6 promoter (dusp6 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-RB 1-602

    Plasmid

    #16006

    Depositor

    Olimpia Meucci

    Article

    Khan et al Cell Death Differ. 2008 Oct . 15(10):166

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 1-602

    Promoter

    Available Since

    June 19, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-Bgh-GFP-Farpl

    Plasmid

    #115501

    Purpose

    expression of rat Farp1

    Depositor

    Thomas Biederer

    Article

    Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

    Insert

    Farp1 (Farp1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available Since

    Dec. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SFG.CNa12_opt.IRES.eGFP

    Plasmid

    #22490

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin A mutant 12 (PPP3CA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    T351E; L354A

    Promoter

    Available Since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. deltaPYD-NLRP11.MYC

    Plasmid

    #131197

    Purpose

    mammalian expression

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    deltaPYD-NLRP11 (NLRP11 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    deletion of the pyrin domain (amino acids 1-124 m…

    Promoter

    pCMV

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1/3xmyc_NLRP11-LRR

    Plasmid

    #131200

    Purpose

    mammalian expression

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    LRR of NLRP11 (NLRP11 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    contains only the leucine-rich repeats (LRRs), am…

    Promoter

    pCMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SFG.CNa22_opt.IRES.eGFP

    Plasmid

    #22491

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin A mutant 22 (PPP3CA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    V314R; Y341F

    Promoter

    Available Since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-hFAM 3B-His

    Plasmid

    #172192

    Purpose

    Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium

    Depositor

    Edward De Robertis

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Insert

    hFAM 3B
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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