We narrowed to 59,426 results for: sap

1. pLenti-gRNA-puro

   Plasmid

   #180426

   Purpose

   Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

   Insert

   gRNAscr
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HsSPHK2

   Plasmid

   #118092

   Purpose

   Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, 10

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJ414-PCY1

   Plasmid

   #98150

   Purpose

   Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST myc MOV10 K530A

   Plasmid

   #51719

   Purpose

   plasmid expresses myc-tagged MOV10 K530A

   Depositor

   Markus Landthaler

   Article

   Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

   Insert

   MOV10 K530A (MOV10 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   K530A

   Promoter

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pN1-mCherry-NKD2

   Plasmid

   #176641

   Purpose

   Expresses NKD2-mCherry fusion protein in mammalian cells

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Tags

   mCherry tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDEST myc MOV10 D645N

   Plasmid

   #51720

   Purpose

   plasmid expresses myc-tagged MOV10 D645N

   Depositor

   Markus Landthaler

   Article

   Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

   Insert

   MOV10 D645N (MOV10 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   D645N

   Promoter

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCellFree_G03 MAPK13

   Plasmid

   #67077

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   MAPK13 (MAPK13 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. IRF3-V5

   Plasmid

   #169775

   Purpose

   Express IRF3 in mammalian cells

   Depositor

   Sung-Liang Yu

   Article

   Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

   Insert

   Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1 (IRF3 Human)
   

   Use

   Tags

   V5 tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. WNV strain-NY99 NS2B-NS3 protease fusion with non cleavable linker with mutation K to A to reduce autoproteolysis

   Plasmid

   #228660

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from West Nile virus with an H163A - catalytically inactive mutation for xxxxx

   Depositor

   Frank von Delft, ASAP Discovery

   Insert

   West Nile portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   H163A - catalytically inactive/West Nile Virus NS…

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pAPM-miR30-MOV10-ts3

    Plasmid

    #174279

    Purpose

    MOV10 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MOV10 shRNA (MOV10 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac-Dual-GST-TEV-SINTBAD-mCherry

    Plasmid

    #208874

    Purpose

    For expression in insect cells of SINTBAD-mCherry

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MAC_N_ELK4

    Plasmid

    #187745

    Purpose

    MAC-tagged gene expression of human ELK4

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ELK4 (ELK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pN1mcherry-NKD2S31A

    Plasmid

    #174332

    Purpose

    encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    mCherry tag

    Expression

    Mammalian

    Mutation

    changed Serine 31 to Alanine

    Promoter

    CMV

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3.1-NKD2S31A

    Plasmid

    #174331

    Purpose

    encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
    

    Use

    Tags

    MycHis

    Expression

    Mammalian

    Mutation

    changed Serine 31 to Alanine

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGS1112

    Plasmid

    #63784

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eSapphire-Strep-tag II followed by URA3
    

    Use

    E. coli cloning vector

    Tags

    Sapphire-Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1a-hMLH1dn

    Plasmid

    #174824

    Purpose

    Mammalian expression of human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-n1-APP

    Plasmid

    #69924

    Purpose

    mammalian expression of human APP fused to EGFP

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    APP (APP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-HsPeg10rc3

    Plasmid

    #174859

    Purpose

    Expresses recoded human Peg10 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    recoded Homo sapiens PEG10 (PEG10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T1_ATG4B_BamHI_NotI

    Plasmid

    #190862

    Purpose

    Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG4B (ATG4B Human)
    

    Use

    Tags

    GST, Thrombin Cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRXIP VPS16-GFP

    Plasmid

    #67023

    Purpose

    Human VPS16 ORF was inserted into pMRXIP GFP-N vector

    Depositor

    Noboru Mizushima

    Article

    Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

    Insert

    Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only