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pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

Plasmid

#218767

Purpose

encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-pAAFRS-9

Expression

Bacterial

Mutation

Y37G, L71V, D182C, F183Y, L186C, D265R

Promoter

tacI

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-NLuc

Plasmid

#135953

Purpose

Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

2xSV40-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-mCherry

Plasmid

#65279

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti Dest BlastR R1-R2

Plasmid

#84574

Purpose

Gateway destination vector expressing Blasticidin resistance under a PGK promoter

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Type

Empty backbone

Use

Lentiviral; Gateway

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304_zeo_empty

Plasmid

#160093

Purpose

Control lentiviral vector for mammalian gene expression

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGW03 AAV-TBG-Cre-sgRNA

Plasmid

#192163

Purpose

AAV-TBG-Cre-sgRNA

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

Plasmid

#40810

Depositor

Natalie Ahn

Article

Mansour et al Science. 1994 Aug 12;265(5174):966-70.

Insert

MAP2K1 (MAP2K1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(31-51), S218E, S222D

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-beta-catenin-IRES-GFP

Plasmid

#14717

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

beta-catenin (Ctnnb1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG J591-scFV T-CAR

Plasmid

#171961

Purpose

Donor plasmid for J591 T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic J591-scFV CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-neo beta catenin S33Y

Plasmid

#16519

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Expression

Mammalian

Mutation

S33Y (SW48 mutation)

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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Lifeact-7-iRFP670

Plasmid

#103032

Purpose

Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Tags

iRFP670

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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RBD-Fc

Plasmid

#164221

Purpose

Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

Spike RBD (S SARS-CoV)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLX302 GDF11-V5 puro

Plasmid

#83097

Purpose

Lentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

GDF11 (GDF11 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pBABE YAP1

Plasmid

#15682

Depositor

Joan Brugge

Article

Overholtzer et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-Src(K295R)

Plasmid

#215465

Purpose

Dox-inducible lentiviral transfer vector with Src kinase-dead mutant (K295R)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Lentiviral

Mutation

K295R (Kinase dead)

Promoter

mCMV, Doxycycline-inducible

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLX317-VRK1

Plasmid

#199643

Purpose

Expresses VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-neo beta catenin delta45

Plasmid

#16520

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

beta catenin (CTNNB1 Human)

Expression

Mammalian

Mutation

Delta 45 (HCT116 mutation)

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pMP71-super-IL-2

Plasmid

#174599

Purpose

Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1-GSK3β-#2

Plasmid

#32497

Depositor

Alex Toker

Article

Yoeli-Lerner et al Mol Cancer Res. 2009 Mar;7(3):425-32. Ep

Insert

GSK3B (GSK3B Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only