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pSH231-EF1-GFP-HYGRO

Plasmid

#115144

Purpose

Safe harbor site 231 knock-in vector with GFP-HygromycinR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Publication

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

GFP

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCWXPGR-pTF-dnTCF4

Plasmid

#114277

Purpose

TET-inducible expression of a dominant negative form of the TCF4 transcription factor

Depositor

Patrick Salmon

Publication

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

TCF4 (TCF4 Human)

Use

Lentiviral

Mutation

deletion of AA 1 to 31

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q19

Plasmid

#111741

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q19 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q23

Plasmid

#111723

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q23 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSLIK CA RhoA

Plasmid

#112894

Purpose

promoter-inducible expression of constitutively-active RhoA

Depositor

Sanjay Kumar

Publication

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

CA RhoA (RHOA Human)

Use

Lentiviral

Expression

Bacterial

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn-Htt171-66Q-myc-WPRE

Plasmid

#107934

Purpose

Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

Depositor

Johan Jakobsson

Publication

Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

Insert

Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)

Use

AAV

Promoter

hSynapsin

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S

Plasmid

#106186

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-HF1RA-P2A-GFP-PGK-Puro

Plasmid

#110866

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBXNPHM3

Plasmid

#110099

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

Depositor

Markus Seeger

Publication

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pJJB308

Plasmid

#107699

Purpose

AtU6 Promoter-LwaCas13a-gRNA Scaffold

Depositor

Daniel Voytas

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Type

Empty backbone

Expression

Plant

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LentiRCas9-CUG

Plasmid

#104183

Purpose

Lentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

Depositor

Eugene Yeo

Publication

Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

Insert

U6-CUGsgRNA, EFS-PIN-dCas9

Use

CRISPR and Lentiviral

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-mCherry-LKB1(WT)

Plasmid

#84639

Purpose

mCherry-tagged, wild-type LKB1

Depositor

Jin Zhang

Publication

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-LBK1 (STK11 Synthetic, Human)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn1-tdTomatof

Plasmid

#104060

Purpose

Also known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracing

Depositor

Viviana Gradinaru

Publication

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

tdTomato

Use

AAV

Promoter

hSyn

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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ecAT3.10

Plasmid

#107215

Purpose

Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

Depositor

Mathew Tantama

Publication

Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecAT3.10

Expression

Mammalian

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

Plasmid

#106204

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S + mRuby3 fusion

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-CAAX

Plasmid

#87612

Purpose

Plasma membrane-targeted Venus (YFP).

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TVT7R(0,0)

Plasmid

#98631

Purpose

Vector for cloning infectious RNA virus genomes

Depositor

Andrew Ball, Karyn Johnson

Publication

Johnson et al J Virol. 2000 Jun;74(11):5123-32.

Type

Empty backbone

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_LIG1_WT_V5

Plasmid

#83006

Purpose

Gateway Donor vector containing LIG1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

LIG1 (LIG1 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(WT)-mCherry

Plasmid

#79422

Purpose

Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Arf6 (ARF6 Human)

Tags

mCherry

Expression

Mammalian

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Publication

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Expression

Bacterial

Promoter

PxylA (B. megaterium)

Available since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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