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SPLICS NU-MT P2A

Plasmid

#210129

Purpose

Detect the Nucleus-Mitochondria contact

Depositor

Tito Calì

Article

Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

Insert

SPLICS NU-MT P2A

Expression

Mammalian

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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ACE2-IgFc probe

Plasmid

#184787

Purpose

Expression of His-tagged, soluble ACE2-IgFc microbody

Depositor

Ajit Chande

Article

Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

Insert

hACE2-IgFc (ACE2 Synthetic)

Tags

6xHis Tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_ORI_neg.cont

Plasmid

#99315

Purpose

Negative control for luciferase validation vectors

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

neutral region; chr3: 55198740-55200041

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMV361_MjTyrRS

Plasmid

#193250

Purpose

Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

Depositor

Peter Schultz, Jessica Seeliger

Article

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Insert

MjTyrRS

Expression

Bacterial

Promoter

hsp60

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MLF2-GFP-3xFLAG

Plasmid

#230986

Purpose

Expresses GFP tagged MLF2 in mammalian cells

Depositor

Christian Schlieker

Article

Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

Insert

MLF2 (MLF2 Human)

Tags

3xFLAG and GFP

Expression

Mammalian

Promoter

T7

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/GFPCD63

Plasmid

#231709

Purpose

Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

Insert

CD63 (CD63 Human)

Tags

gfp

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-foldon-GFP- PCP

Plasmid

#194503

Purpose

Expression foldon-GFP-PCP protein for imaging of nonrepetitive DNA

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Insert

foldon-GFP-PCP

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-IP- SCAT3.2

Plasmid

#172497

Purpose

SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.

Depositor

David Scadden

Article

Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

Insert

Amcyan

Use

Retroviral

Tags

mVenus

Expression

Mammalian

Promoter

MLV LTR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9-HF1

Plasmid

#138556

Purpose

Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-HF1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-Sox10

Plasmid

#36978

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox10 (SOX10 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

Plasmid

#100855

Purpose

AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

jRGECO1b

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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0_T7-mScarlet3

Plasmid

#225927

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-LGALS3

Plasmid

#175756

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-cmv-SpCas9-TadCBEd-V106W-BlastR

Plasmid

#193849

Purpose

Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadCBEd-V106W

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PTGDR-DuET

Plasmid

#213369

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

PTGDR (PTGDR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-oA ONE-GO

Plasmid

#189732

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-oA ONE-GO
GNAO (Gnao1 Rat)

Use

Lentiviral

Mutation

I250 (silent mutation in YFP)

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEIF3D-WT-sgNT

Plasmid

#222120

Purpose

EIF3D overexpression vector with non-targeting sgRNA

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

Plasmid

#201683

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pF709-pET28a-Hs-FL-ETFbeta-NHis wt

Plasmid

#85110

Purpose

expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length ETFbeta (ETFB Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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