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MSI2-DCD

Plasmid

#166968

Purpose

Express the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADAR

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-DCD

Use

Retroviral

Tags

MSI2-DCD

Expression

Mammalian

Mutation

N495S

Promoter

MSCV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT077

Plasmid

#180511

Purpose

Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
PlmCasX

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER13 (miR-LUP)

Plasmid

#46936

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCA13-pHR-SFFV-HaloTag-FBL

Plasmid

#199444

Purpose

Expression of HaloTag-FBL in mammalian cells

Depositor

Baohui Chen

Article

Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

Insert

FBL (FBL Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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5_T7-GAP43-mScarlet3

Plasmid

#225932

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

GAP43-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAdEasy-EGFP-CLASP2 340-1362

Plasmid

#24522

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40

Plasmid

#100846

Purpose

AAV expression of Cre recombinase-activated jRCaMP1a, a red fluorescent calcium sensor protein, from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRCaMP1a

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 bovine GRK5

Plasmid

#14690

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK5 (GRK5 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
AAV-FLEX-PKIalpha-IRES-nls-mRuby2

Plasmid

#63059

Purpose

Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

PKIalpha (Pkia Mouse, Synthetic)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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miniABEmax (pRZ900)

Plasmid

#131311

Purpose

CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-HA-hRb-WT-T373A-S608A-S612A-puro

Plasmid

#212674

Purpose

Express tagged hRb mutant

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Mutation

T373A/S608A/S612A

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-MAP1LC3BK51A

Plasmid

#175754

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B K51A

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Mutation

K51A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-hNF2

Plasmid

#107150

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-HA-GOLPH3

Plasmid

#21687

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-GFP

Plasmid

#28307

Purpose

Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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PIK3C2B gRNA (BRDN0001149054)

Plasmid

#76712

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3C2B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3C2B (PIK3C2B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMIA10.53-Clover

Plasmid

#214450

Purpose

BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

mClover

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaX

Plasmid

#176121

Purpose

Constitutivley active, mitochondrial localized Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

CDC42 (CDC42 Human)

Expression

Mammalian

Mutation

G12V

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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ABE8.13-m

Plasmid

#136296

Purpose

expresses ABE8.13-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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