We narrowed to 65,929 results for: des.1

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TFORF3473

Plasmid

#144949

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-TGFB1

Plasmid

#164427

Purpose

AAV plasmid expressing TGFB1 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb1 (Tgfb1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Nov. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD9-pHuji

Plasmid

#130906

Purpose

Expression of CD9-pHuji for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD9-pHuji (CD9 Synthetic, Human)

Tags

pHuji

Expression

Mammalian

Mutation

pHuji inserted between Asn50 and Asn51 in extrace…

Promoter

CMV

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5myc Rac1 wt

Plasmid

#37030

Depositor

Lawrence Kirschner

Article

Manchanda et al Oncogene. 2013 Jul 25;32(30):3491-9. doi

Insert

Rac1 (RAC1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
PYCR1

Plasmid

#38842

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PYCR1 (PYCR1 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV-fly Dicer

Plasmid

#171559

Purpose

Encodes fly Dicer protein

Depositor

Yan Zeng

Insert

Dcr-1(CG4792) (Dcr-1 Fly)

Expression

Mammalian

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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PxylA

Plasmid

#55178

Purpose

Xylose-inducible promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

xylA promoter

Use

Synthetic Biology; Bacillus biobrick box

Promoter

xylA

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCMU-MTUBr

Plasmid

#61196

Purpose

Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MAP-MBD

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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FlucSM-HA-GFP11-N1

Plasmid

#91955

Purpose

Expresses aggregation-prone FlucSM-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucSM-HA-GFP11

Expression

Mammalian

Mutation

R188Q

Promoter

CMV

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flt3-ITD-Y591F/Y919F

Plasmid

#74499

Purpose

Retroviral expression of FLT3 ITD-Y591F/Y919F

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

unknown

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCre-SacB-zeo

Plasmid

#107706

Purpose

Expresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

ble (Zeo resistance)

Expression

Bacterial

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-R2Tg_opt-3xFlag

Plasmid

#223251

Purpose

Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg protein_opt

Tags

Flag

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRClover

Plasmid

#59142

Purpose

ORF encoding p38 KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

Plasmid

#25846

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA-W37I

Tags

Flag and His

Expression

Mammalian

Mutation

W37I

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.HA.RUNX1C.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#181978

Purpose

Human RUNX1C gene overexpression

Depositor

Jan-Henning Klusmann

Article

Gialesaki et al Blood. 2023 Mar 9;141(10):1105-1118. doi

Insert

RUNX1C (human) (RUNX1 Human)

Use

Lentiviral

Tags

HA (Human influenza hemagglutinin)

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

Plasmid

#154780

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

CRFR1 (Crhr1 Rat)

Tags

HA

Expression

Mammalian

Mutation

95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry

Plasmid

#108685

Purpose

Strong CAG promoter with mCherry expression for transfection efficacy screening

Depositor

Jordan Green

Article

Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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