We narrowed to 9,085 results for: mel

1. NpT7-5-ERK1

   Plasmid

   #39229

   Depositor

   Melanie Cobb

   Article

   Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

   Insert

   ERK1 (MAPK3 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pOPS0359

   Plasmid

   #115508

   Purpose

   Reporter Plasmid. Taurine-inducible expression of GFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Taurine-inducible promoter of S. meliloti Promote…

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL1.7XX

   Plasmid

   #49344

   Purpose

   luciferase reporter with mouse Bmp2 promoter

   Depositor

   Melissa Rogers

   Article

   Abrams et al J Biol Chem. 2004 Apr 16;279(16):15916-2

   Insert

   Bmp2 distal promoter (Bmp2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Mutation

   nt –1237 to +471

   Promoter

   Bmp2 distal promoter

   Available Since

   Dec. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM263-EcPPXc

   Plasmid

   #38328

   Depositor

   Florian Freimoser

   Article

   Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

   Insert

   E. coli polyphosphatase (ppx Escherichia coli)
   

   Tags

   6xHIS, GST, and TEV

   Expression

   Bacterial

   Mutation

   C-terminus of E. coli polyphosphatase (described …

   Promoter

   T7

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-mClover3

   Plasmid

   #171497

   Purpose

   T7 promotor drives in vitro transcription of mClover3 mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   mClover3
   

   Use

   Pgehme

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-WingGFP-tan

   Plasmid

   #83473

   Purpose

   EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   WingGFP-tan
   

   Use

   CRISPR

   Expression

   Bacterial and Insect

   Promoter

   Hsp70 and enhancer from D. melanogaster yellow ge…

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-His-Flag-Tipin

   Plasmid

   #22890

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Tipin (TIPIN Human)
   

   Tags

   Flag- and His

   Expression

   Insect

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

8. EPHA7 (3NRU)

   Plasmid

   #28126

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHA7:SDC173-D03:C209131 (EPHA7 Human)
   

   Tags

   His and Honeybee melittin Signal sequence (MKFLVN…

   Expression

   Bacterial

   Mutation

   contains amino acids residues 32-204.

   Available Since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pBABE.puro/TO/Flag/tGFP.HuR.S221D

   Plasmid

   #110351

   Purpose

   Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tags

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   S221D, P237S

   Promoter

   gag

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPS833

    Plasmid

    #1358

    Depositor

    Pamela Silver

    Article

    Schlenstedt et al Proc Natl Acad Sci U S A 1995 Jan 3;92(1

    Insert

    GSP1 (GSP1 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    G21V, stabilizes the GTP-bound form

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pJK429

    Plasmid

    #155376

    Purpose

    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASt-ssBiP-ESCargo*

    Plasmid

    #140165

    Purpose

    Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssBip-DsRed-Express2-FKBP(LV)(C22V)
    

    Expression

    Insect

    Promoter

    hs70

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNAintron-LASV-GPC-c3xFLAG

    Plasmid

    #215689

    Purpose

    Produces the Lassa virus glycoprotein with a c-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

    Insert

    LASV GP-FLAG
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pnYC-NvHN-DmNot4_813-836opt_AG

    Plasmid

    #148793

    Purpose

    Bacterial Expression of DmNot4_813-836opt

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    DmNot4_813-836opt (CG31716 Synthetic)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ppwd1.473.646.W601A

    Plasmid

    #137660

    Purpose

    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

    Depositor

    Tara Davis, Melissa Jurica

    Article

    Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

    Insert

    ppwd1 (PPWD1 Human)
    

    Tags

    (His)6-thrombin

    Expression

    Bacterial

    Mutation

    473-646-W601A

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE.puro/TO/Flag/HuR.S221D

    Plasmid

    #110347

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG tag

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S221D, P237S

    Promoter

    gag

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABE.puro/TO/Flag/HuR.S221D

    Plasmid

    #110355

    Purpose

    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and mKO2 tags

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and mKO2

    Expression

    Mammalian

    Mutation

    S221D, P237S

    Promoter

    gag

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)

    Plasmid

    #84661

    Purpose

    mammalian expression and/or retrovirus preparation

    Depositor

    Ayano Satoh

    Article

    Toyota et al Sciencematters

    Insert

    Laforin (EPM2A Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Nov. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pQCXIP-Laforin Myc 3CS_new (C266S, C278S, C329S)

    Plasmid

    #84662

    Purpose

    mammalian expression and/or retrovirus preparation

    Depositor

    Ayano Satoh

    Article

    Toyota et al Sciencematters

    Insert

    Laforin (EPM2A Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    5'LTR

    Available Since

    Nov. 24, 2016

    Availability

    Academic Institutions and Nonprofits only