We narrowed to 9,399 results for: pho;

1. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

   Plasmid

   #26966

   Purpose

   AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

   Depositor

   Karl Deisseroth

   Article

   Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   eNpHR 3.0
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   ER export at 3' end of gene. Trafficking sig…

   Promoter

   EF1alpha

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKeima-LiveDrop

   Plasmid

   #199695

   Purpose

   lipophagy reporter system based on the Keima-fluorophore

   Depositor

   Tobias Walther

   Article

   Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

   Insert

   GPAT4 (Gpat4 Fly)
   

   Tags

   Keima

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NEK7_HUMAN_D0

   Plasmid

   #79735

   Purpose

   This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   NEK7 (NEK7 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. psicheck2 PTEN 3'UTR

   Plasmid

   #50936

   Purpose

   reporter for PTEN RNAi

   Depositor

   Pier Pandolfi

   Article

   Tay et al Cell. 2011 Oct 14;147(2):344-57. doi: 10

   Insert

   PTEN 3'UTR (PTEN Human)
   

   Use

   RNAi

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Tagged-IRES

   Plasmid

   #35570

   Depositor

   Marian Waterman

   Article

   Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

   Insert

   Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase and MS2 Stem Loop

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-CaMPARI

   Plasmid

   #60421

   Purpose

   Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI
   

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PIK3CA - Exon20 H1047R

   Plasmid

   #16639

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Science. 2004 Apr 23. 304(5670):554.

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H1047R

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ609-dCas9-KR-puro

   Plasmid

   #158478

   Purpose

   Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

   Depositor

   Brendan Price

   Article

   House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

   Insert

   dCas9-KR
   

   Use

   CRISPR

   Tags

   KillerRed

   Expression

   Mammalian

   Mutation

   Cas9m4 (D10A+D839A+H840A+N863A)

   Promoter

   CMV

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-FOXM1

   Plasmid

   #153136

   Purpose

   Expression of Flag tagged human FOXM1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXM1 (FOXM1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-MbPylRS(DiZPK)

    Plasmid

    #91706

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in mammalian cells to incorporate the photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Lin et al Angew Chem Int Ed Engl. 2013 Dec 23;52(5

    Inserts

    pylS
    pylT
    

    Expression

    Mammalian

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PyronicSF/pcDNA3.1(-)

    Plasmid

    #124812

    Purpose

    Highly resposive GFP-based pyruvate nanosensor.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)mGAT1-0-GFP

    Plasmid

    #41662

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal hydrophobic isoleucine residue added a…

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-CALCOCO1

    Plasmid

    #137765

    Purpose

    Expression and localization of tagged CALCOCO1

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    CALCOCO1 (CALCOCO1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PyronicSF-mRuby2/pBI-CMV1

    Plasmid

    #124830

    Purpose

    Highly resposive GFP-based pyruvate nanosensor.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Inserts

    PyronicSF
    mRuby2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

    Plasmid

    #115516

    Purpose

    Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

    Depositor

    Marco Morsch

    Article

    Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

    Insert

    MLS-KillerRed
    

    Use

    Gateway cloning

    Tags

    MLS

    Promoter

    UAS

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GPD-roGFP

    Plasmid

    #49436

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    GPD-roGFP2
    

    Use

    Adenoviral

    Tags

    Glycerol phosphate dehydrogenase, 5' to roGF…

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available Since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD miniSOG

    Plasmid

    #91774

    Purpose

    Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    mini Singlet Oxygen Generator
    

    Tags

    Hexa-Histidine tag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVAX1-BMP2

    Plasmid

    #137909

    Purpose

    Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

    Depositor

    Georg Feichtinger

    Article

    Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

    Insert

    BMP2 (BMP2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFPC1-FLAG-Synaptojanin 1-145

    Plasmid

    #22293

    Depositor

    Pietro De Camilli

    Article

    Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

    Insert

    Synaptojanin 1_145 (SYNJ1 Human)
    

    Tags

    Flag and GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. PAmCherry-N1-FRB-Tubbyc

    Plasmid

    #202678

    Purpose

    Photoactivatable and rapamycin recruitable lipid biosensor

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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