We narrowed to 16,584 results for: EMB

1. 6xHis-TEV-UBC-9

   Plasmid

   #87055

   Purpose

   Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

   Depositor

   Ronald Hay

   Article

   Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

   Insert

   UBC-9 (ubc-9 Nematode)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Oct4-Cr1

   Plasmid

   #66989

   Purpose

   Expresses gRNA that targets stop codon for OCT4

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

   Insert

   gRNA for Crispr targeting the OCT4 stop codon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pLV-EF1a-V5-Sec61B

   Plasmid

   #120244

   Purpose

   Expresses V5-tagged Sec61B in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Sec61B (Sec61b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C-BLa 2.0

   Plasmid

   #92060

   Purpose

   The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.

   Depositor

   Dieter Langosch

   Article

   Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

   Insert

   C-BLa 2.0 fusion protein
   

   Use

   Tags

   Flag (DYKDHDG)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6-26 (GB1001)

   Plasmid

   #68208

   Purpose

   Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtU6-26 promoter
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3 Flag Jnk1b2

   Plasmid

   #13752

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk1b2 (MAPK8 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pGST1-GGA3-VHS

   Plasmid

   #44420

   Depositor

   James Hurley

   Article

   Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

   Insert

   GGA3-VHS (GGA3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-CAG-miniSOG-T2A-dTomato

   Plasmid

   #193796

   Purpose

   Expresses miniSOG in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   miniSOG
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE80L TriCatcher-RGDSP-MMP

   Plasmid

   #112633

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-RGDSP-MMP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central matrix metalloproteinase-cleavable sequen…

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGB Tnos:NptII:Pnos (GB0226)

    Plasmid

    #68212

    Purpose

    Transcriptional unit for kanamycin resistance gene plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    NptII
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pL0_EF-H4

    Plasmid

    #202178

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    H4 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pL0_EF-fcpA

    Plasmid

    #202164

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    fcpA terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pL0_AC-H4

    Plasmid

    #202122

    Purpose

    Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    H4 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pL0_AC-P49

    Plasmid

    #202120

    Purpose

    Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    P49 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCMV-BD-cop1at(335-675)

    Plasmid

    #44973

    Depositor

    Thanos Halazonetis

    Article

    Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

    Insert

    BD-COP1 (COP1 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-334

    Promoter

    pCMV

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKG209

    Plasmid

    #45109

    Depositor

    Iain Cheeseman

    Article

    Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

    Insert

    CENP-T (aa1-374) Laci-NLS (CENPT Human)
    

    Use

    Tags

    GFP, Laci-NLS, s peptide, and tev

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHsp18.2 (GB0153)

    Plasmid

    #68171

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAC-mCherry2-G4

    Plasmid

    #164167

    Purpose

    Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEasyG1_zeo

    Plasmid

    #184910

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-EGFP-ERas-IP

    Plasmid

    #13826

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

    Insert

    ERas (Eras Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only