We narrowed to 24,484 results for: promoter
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Plasmid#162791PurposeMultiplex Guide RNAs to generate Nr4a1 knockout by CRISPRDepositorAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only
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LacI-pLacI-pIII pm
Plasmid#218100PurposePhagemid that expresses LacI, and pIII under the Lac promoterDepositorInsertpIII
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-sgH11-eCas9-T2A-BlastR
Plasmid#172492PurposeFor CRISPR-assisted HDR, Hipp11 locusDepositorInsert, BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
UseCRISPR, Lentiviral, and Mouse TargetingAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PLVX_SPTA1_IGF2BP1_IRES_PURO
Plasmid#100016PurposeTo over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.DepositorInsertInsulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
UseLentiviralExpressionMammalianPromoterSPTA1 erythroid specific promoterAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
miniCRISPRoff-v1
Plasmid#197030PurposeVector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoterDepositorInserthumanized miniCRISRPoff-v1
ExpressionMammalianMutationD52R / T132R / D228A / D406APromoterCBh, CMV, hU6Available SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-HsBIN1_tv8
Plasmid#176012PurposeMammalian expression of Human EGFP-Bin1 (transcriptional variant 8, muscle specific). Parton lab clone HBHDepositorInsertBin1
TagsEGFPExpressionMammalianAvailable SinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-ChR2-EYFP
Plasmid#183765PurposeThis plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.DepositorInsertAAV transgene -teto promoter-flex/dio-ChR2-EYFP
UseAAV, Cre/Lox, and Mouse TargetingTagsChR2-EYFPExpressionMammalianPromotertetO promoterAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Chronos-tdTomato
Plasmid#62726PurposeAAV-mediated expression of Chronos-tdTomato under the Syn promoter. Using bGHpA signal. tdTomato has codons varied between the first and second tandem repeats to reduce recombination.DepositorHas ServiceAAV8InsertChronos-tdTomato
UseAAVTagstdTomatoExpressionMammalianPromoterhuman synapsin promoterAvailable SinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6-sgfiller-eCas9-T2A-BlastR
Plasmid#172437PurposeExpresses eCas9, BlastR and sgRNA cloning siteDepositorInsertseCas9
BlastR
UseCRISPR, Lentiviral, and Mouse TargetingAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
Plasmid#171381PurposeExpression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized Cas13Y.1
Tags3xFLAGExpressionMammalianPromoterCMV, U6Available SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG_FBXL7
Plasmid#171848PurposepcDNA3-FLAG_FBXL7DepositorAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-hBVRA
Plasmid#100285PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cas12Max
Plasmid#195334Purposevector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized Cas12Max
Tags3xFlagExpressionMammalianMutationN243RPromoterCBh, CMV, hU6Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
gCBEv2
Plasmid#220617Purposevector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInserthUNG2-nSpCas9
ExpressionMammalianMutationhUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)PromoterhU6, EF1aAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hLin28B
Plasmid#26358DepositorAvailable SinceNov. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)
Plasmid#78507PurposeConstruct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labelingDepositorInsertGads SH3 SH2 SH3 domain (GRAP2 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pIV pm
Plasmid#218101PurposePhagemid that expresses LacI, and pIV under the Lac promoterDepositorInsertpIV
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS_128
Plasmid#160651PurposeExpresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e3 (APOE Human)
TagsKar2 signal sequenceExpressionYeastMutatione3 allelePromoterpZ estradiol inducible promoterAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCELF4-4405
Plasmid#115466PurposeConstitutive lentiviral expressionDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUNO mouse CD274 + 3'UTR WT full length
Plasmid#107012PurposeExpresses murine PD-L1 with the 3'UTRDepositorInsertCD274 (Cd274 Mouse)
ExpressionMammalianAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only