We narrowed to 14,443 results for: NTS

1. pSLQ9715: pHR-PGK-SV40_NLS-dCasMINI-V4-NFZ-c-Myc_NLS-mCherry-WPRE

   Plasmid

   #203221

   Purpose

   Expression of dCasMINI-V4-NFZ-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCasMINI-V4-NFZ-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. GB1-A11(2-196)-Strep

   Plasmid

   #196208

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SaPE2

   Plasmid

   #174817

   Purpose

   Mammalian expression of SaCas9 prime editor 2

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   SaPE2
   

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-h53BP1 (siRNA resistant)

   Plasmid

   #110301

   Purpose

   Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

   Depositor

   Chris Kok-Lung Chan

   Article

   Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   GAACGAGGA to GAGCGGGGC

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-hygro p53 DD

   Plasmid

   #9058

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   p53 DD (Trp53 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   DD. Dominant negative mutant carries deletion of…

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

   Plasmid

   #139992

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   VRQR=D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-LRP6-3XFlag

   Plasmid

   #180144

   Purpose

   flag tagged LRP6

   Depositor

   Edward De Robertis

   Article

   Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

   Insert

   Human LRP6 (LRP6 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

   Plasmid

   #171594

   Purpose

   Strong human ACE2 abundance from Bxb1 landing pad

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   ACE2 (ACE2 Human)
   mCherry
   H2A
   Pac
   

   Use

   Synthetic Biology; Promoterless bxb1 dna recombin…

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2 LRP6-eGFP

   Plasmid

   #180143

   Purpose

   to make movies of LRP6 vesicles

   Depositor

   Edward De Robertis

   Article

   Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

   Insert

   Human LRP6 (LRP6 Human)
   

   Expression

   Mammalian

   Promoter

   SP6

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.2-FUS-1-526aa-V5

    Plasmid

    #29609

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR EGFR-CD3ε-FusionRed

    Plasmid

    #188626

    Purpose

    Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

    Depositor

    Jared Toettcher

    Article

    Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

    Insert

    EGFR (EGFR Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    CD3ε-FusionRed

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA6.2EmGFP-DAXX

    Plasmid

    #169726

    Purpose

    Mammalian expression construct of GFP tagged full length DAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    DAXX (DAXX Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MtPT4-p3

    Plasmid

    #160003

    Purpose

    Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Inserts

    DsRed (reverse orientation)
    DODAα1
    CYP76AD1
    cDOPA5GT
    

    Expression

    Plant

    Promoter

    Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5_mCherry-CRM1

    Plasmid

    #237646

    Purpose

    For overexpression of mCherry-CRM1

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    CRM1 (XPO1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV hSYN DIO mGluR1-myc-FLAG

    Plasmid

    #166227

    Purpose

    AAV expressing cre-dependent mGluR1-myc-FLAG

    Depositor

    Jason Aoto

    Article

    Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

    Insert

    metabotropic glutamate receptor 1 (Grm1 Mouse)
    

    Use

    AAV and Cre/Lox

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139090

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1 Utr230-EGFP-3XNLS

    Plasmid

    #58466

    Purpose

    Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Inserts

    Utr230 with 3X glycine linker (UTRN Human)
    3X NLS with 3X stop codons
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-eNme2-T.1-ABE8e

    Plasmid

    #185668

    Purpose

    Expresses eNme2-T.1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFPC1-muscle Amphiphysin 2

    Plasmid

    #22213

    Depositor

    Pietro De Camilli

    Article

    Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

    Insert

    Amphiphysin II (BIN1 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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