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pAAV_S34F_U2AF1

Plasmid

#176592

Purpose

Adenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

U2AF1 (U2AF1 Human)

Use

AAV

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBA52_Ubiquitin

Plasmid

#110043

Purpose

Protein expression and purification of UBA52_Ubiquitin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UBA52_Ubiquitin (UBA52 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_C_INSR

Plasmid

#187772

Purpose

MAC-tagged gene expression of human INSR

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

INSR (INSR Human)

Expression

Mammalian

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par3b

Plasmid

#19388

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 3b (PARD3 Human)

Tags

Myc

Expression

Mammalian

Mutation

Full length, isoform b.

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-TARG1

Plasmid

#172586

Purpose

For transient expression of N-terminal GFP-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28c-F30-2xdBroccoli-47CAG

Plasmid

#237217

Purpose

Express F30-2xdBroccoli tagged 47 repeats of CAG RNA in bacteria.

Depositor

Mingxu You

Article

Xue et al Nucleic Acids Res. 2023 Sep 8;51(16):833

Insert

47 CAG trinucleotide repeats RNA

Expression

Bacterial

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_009

Plasmid

#123672

Purpose

Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1-2xHS4

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL_U2AF1_S34F_mCherry

Plasmid

#84019

Purpose

expression of mutant U2AF1 in mammalian cells

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Mutation

S34F

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Tol2-UAS-Dendra2

Plasmid

#97145

Purpose

For Gal4/UAS expression system

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Dendra2

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-GRM1-IRES-CyRFP1

Plasmid

#195355

Purpose

mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

Depositor

Brenda Bloodgood

Article

Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

Insert

GRM1-IRES-CyRFP1

Expression

Mammalian

Promoter

pCAG

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin

Plasmid

#134662

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 5 (VAMP5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126218

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

Lentiviral

Expression

Mammalian

Promoter

CamKIIa

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2

Plasmid

#27415

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pAR-Ec611

Plasmid

#130872

Purpose

A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRep

Depositor

Chang Liu

Article

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Mutation

I777K, L900S

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET-6His-MBP-MpAgo

Plasmid

#79249

Purpose

This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression

Depositor

Jennifer Doudna

Article

Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p

Insert

Marinitoga piezophila Argonaute

Use

CRISPR

Tags

6XHis-tag + MBP + PreScission Cleavage Site

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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MAC_C_INSR

Plasmid

#187788

Purpose

MAC-tagged gene expression of human INSR

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

INSR (INSR Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pdCas9-J23111

Plasmid

#113149

Purpose

Plasmid constitutively expressing dCas9 protein

Depositor

Chong Zhang

Article

Wang et al Nat Commun. 2018 Jun 26;9(1):2475. doi:

Insert

dCas9

Expression

Bacterial

Mutation

D10A & H840A

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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MTK1_013

Plasmid

#123676

Purpose

Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-2xHS4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-2H12.D11

Plasmid

#221145

Purpose

His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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