159,237 results

1. pHis17_ParM _1

   Plasmid

   #78201

   Purpose

   Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

   Insert

   parM (K33A I27C T174A T175N C287A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A I27C T174A T175N C287A

   Promoter

   unknown

   Available since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZac2.1-GfaABC1D -IgK-TurboID-HA

   Plasmid

   #219810

   Purpose

   Secreted HA-tagged TurboID for extracellular proximity labeling

   Depositor

   Cagla Eroglu

   Article

   Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

   Insert

   IgK (Igk Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDZ50

   Plasmid

   #41948

   Depositor

   Stephen Elledge

   Article

   Zhang et al Cell. 2006 Aug 11;126(3):529-42.

   Insert

   ubiquitin specific peptidase 28 (USP28 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2013

   Availability

   Academic Institutions and Nonprofits only

4. TFORF1906

   Plasmid

   #141883

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF7L2 (TCF7L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetroX SgK269 WT

   Plasmid

   #68028

   Purpose

   expression of Sgk269 in mammalian cells

   Depositor

   Roger Daly

   Article

   Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

   Insert

   PEAK1 (PEAK1 Human)
   

   Use

   Retroviral

   Tags

   FLAG/myc, HA, and IRES-DsRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

6. RCAS-Flag-BRAFV600E

   Plasmid

   #126615

   Purpose

   Retroviral expression of Flag tagged human oncogenic BRAFV600E

   Depositor

   Yi Li

   Article

   Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP13999 - pAAV-AiE0387m-minBG-iCre(R297T)-BGHpA (Alias: CN3999)

   Plasmid

   #208166

   Purpose

   AiE0387m is an enhancer sequence designed to drive AAV-mediated Cre recombination in brain astrocytes

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Insert

   iCreR297T
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

   Plasmid

   #111393

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis, Myc, T7, and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEB1-mYPet

   Plasmid

   #103988

   Purpose

   Plasmid encoding mYPet

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mYPet
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F46L, I47L, …

   Promoter

   proC

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-N BNLF2a

    Plasmid

    #37941

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    BNLF2a (BNLF2a H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available since

    Aug. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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