We narrowed to 12,197 results for: 112

1. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

   Plasmid

   #194681

   Purpose

   CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_01-mEGFP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-CreERT2 stuffer v3

   Plasmid

   #158046

   Purpose

   lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBV65

   Plasmid

   #108363

   Purpose

   A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

   Depositor

   Scott Moye-Rowley

   Article

   Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

   Insert

   loxP-natMX6-CgMET3prom-cre-loxP
   

   Use

   Gene disruption marker for candida glabrata

   Promoter

   C. glabrata MET3

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.11-Cd36-promoter

   Plasmid

   #138572

   Purpose

   Firefly luciferase reporter fused with a 1296 bp fragment of mouse Cd36 promoter

   Depositor

   Lora Hooper

   Article

   Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

   Insert

   Cd36 Promoter (Cd36 Mouse)
   

   Use

   Luciferase

   Promoter

   SV40

   Available Since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-p38alpha

   Plasmid

   #86832

   Purpose

   mammalian expression of GFP-p38alpha

   Depositor

   Rony Seger

   Article

   Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

   Insert

   p38alpha (MAPK14 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

   Plasmid

   #176541

   Purpose

   human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   OgeuIscB omegaRNA scaffold
   

   Expression

   Mammalian

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK-FLAG-UbxD8

   Plasmid

   #53777

   Purpose

   express FLAG-tagged human UbxD8 gene

   Depositor

   Yihong Ye

   Article

   Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

   Insert

   UbxD8 (FAF2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pConRef-2H12.D11

   Plasmid

   #221151

   Purpose

   cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2
   mScarlet-I
   

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2)

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPRv2_mSTING_scrambled_gRNA_1

    Plasmid

    #196627

    Purpose

    Scrambled control 1 for STING knock-out in murine cells.

    Depositor

    Nicolas Manel

    Article

    Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

    Insert

    mSTING scrambled gRNA 1
    

    Use

    CRISPR and Lentiviral

    Mutation

    WT

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-OSBP-PH

    Plasmid

    #49571

    Purpose

    expresses the plekstrin homology domain of OSBP in mammalian cells

    Depositor

    Marci Scidmore

    Article

    Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

    Insert

    OSBP-PH (AA 91-179) (OSBP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-RILPL1

    Plasmid

    #201470

    Purpose

    For overexpression in cultured cells to visualize RILPL1 protein interactions.

    Depositor

    Suzanne Pfeffer

    Article

    Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

    Insert

    RILPL1 (RILPL1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG_IGF1R_EGFP

    Plasmid

    #199628

    Purpose

    FRET donor for IGF1R sensor

    Depositor

    Ryohei Yasuda

    Article

    Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

    Insert

    IGF1R (IGF1R Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-hPR-A

    Plasmid

    #89119

    Purpose

    mammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-A (PGR Human)
    

    Expression

    Mammalian

    Mutation

    human progesterone receptor-A, equivalent to huma…

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. CoV2-N-WT-Hu1

    Plasmid

    #177937

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (original Wuhan Hu1 sequence) (N )
    

    Expression

    Mammalian

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAf-CRISPR-yA

    Plasmid

    #191015

    Purpose

    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

    Insert

    yA
    

    Use

    CRISPR

    Promoter

    Aspergillus flavus U6

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p35S:Citrine-TurboID-3xHA

    Plasmid

    #199243

    Purpose

    This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

    Insert

    Citrine-TurboID
    

    Tags

    3xHA and TurboID-3xHA

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. SNAP-OMP25

    Plasmid

    #141149

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Budding Yeast, Rat)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUBQ:Citrine-TurboID-3xMyc

    Plasmid

    #199244

    Purpose

    This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

    Insert

    Citrine-TurboID-3xMyc
    

    Tags

    TurboID-3xMyc

    Expression

    Plant

    Promoter

    Arabidopsis UBQ10 promoter with intron

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

    Plasmid

    #194683

    Purpose

    CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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