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mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Tags

FRB (T2098L)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBUN6A11

Plasmid

#50579

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

dCas9-VP64
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG, HA, and NLS

Mutation

dCas9 = defective in DNA cleavage; 4×minimal VP16…

Promoter

OsU3p and Ubi1p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET-Expotin 1

Plasmid

#42047

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Exportin-1 (XPO1 Human)

Tags

His

Expression

Bacterial

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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mIFP12-Farnesyl-5

Plasmid

#56251

Purpose

Localization: Plasma Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Farnesyl (HRAS Human)

Tags

mIFP12

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-mScarlet-SARS-CoV-2-SL1 alone

Plasmid

#191485

Purpose

Lentiviral expression of SARS-CoV-2 5_ UTR SL1 alone

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-Puro (pRW1441)

Plasmid

#216217

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

Plasmid

#32627

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Donor + Sce BFP

Use

Lentiviral

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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sgRNA_expression_vector

Plasmid

#210212

Purpose

An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

Depositor

Albert Jeltsch

Article

Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pALPS vif

Plasmid

#101328

Purpose

Encodes codon optimized NL4-3 vif

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 vif

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

Plasmid

#191484

Purpose

Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_Lambda-N-HA-Peptide

Plasmid

#92005

Purpose

Expresses Lambda-N-HA peptide

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

Lambda-N-HA peptide

Tags

Lambda-N-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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C1(1-29)-miniTurbo-V5_pCDNA3

Plasmid

#107174

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR::rpsL

Plasmid

#44505

Purpose

A crRNA expression plasmid specific to the rpsL allele.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR::rpsL (rpsL )

Use

CRISPR; E.coli

Available Since

April 16, 2013

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-KanMx

Plasmid

#122896

Purpose

ADE2-KanMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR1c-V5/HIS

Plasmid

#201106

Purpose

expression of human FGFR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-T2A-Puro

Plasmid

#101039

Purpose

Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

Depositor

Andrea Németh

Type

Empty backbone

Use

CRISPR

Tags

3xFLAG and T2A-Puro

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHO_pTEFmut7_CggR_ble

Plasmid

#124584

Purpose

Plasmid for genomic integration of CggR repressor regulated by the pTEFmut7 promoter

Depositor

Matthias Heinemann

Article

Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

Insert

cggR (cggR Synthetic)

Use

Synthetic Biology

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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