We narrowed to 12,743 results for: CAR

1. pATP416-­ptetO7-­pphlO6-­crtYBI

   Plasmid

   #165976

   Purpose

   Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Inserts

   Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
   Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
   BTS1 (BTS1 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   C1281A, G1698A and C66A, C1359T

   Promoter

   Synthetic promoter (pphlO6), Synthetic promoter (…

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57-Cx46-IRES-GCaMP6s

   Plasmid

   #188238

   Purpose

   3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

   Insert

   GJA3 (GJA3 Human)
   

   Use

   Lentiviral

   Tags

   GCaMp6s

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLRC1-NEK10p:NEK10-3XFLAG

   Plasmid

   #137030

   Purpose

   Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

   Depositor

   David Sabatini

   Article

   Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

   Insert

   NIMA-like kinase 10 (NEK10 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Promoter

   human NEK10 endogenous promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGTag-TagRFP-B-actin

   Plasmid

   #117808

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. WT1 KI gRNA2

   Plasmid

   #92313

   Purpose

   CRISPR-GFP-gRNA for cutting WT1

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Insert

   WT1 (WT1 Human)
   

   Use

   Tags

   CRISPR GFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. INS-GFP-CD19-EPT

   Plasmid

   #210469

   Purpose

   Donor plasmid for knock-in GFP-CD19 into the human INS locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2 Halo-Tev- HSPB7 (zebrafish) WT

   Plasmid

   #108916

   Purpose

   Express WT Zebrafish Halo-Tev-HSPB7 in mammalian cells

   Depositor

   Yimon Aye

   Article

   Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

   Insert

   Halo-TEV-HSPB7 (hspb7 Zebrafish)
   

   Use

   Tags

   Halo-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

   Plasmid

   #179843

   Purpose

   Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP

   Depositor

   Xiaoping Bao

   Article

   Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Luciferase and Synthetic Biology

   Tags

   eGFP and luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

   Plasmid

   #160623

   Purpose

   TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:dCas9:VPR-Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE2-TetOminiCMV-OS

    Plasmid

    #136576

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRepair-mScI-CTNNB1

    Plasmid

    #153431

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    mScarlet-i

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 SARS-CoV-2 S D614G-trunc

    Plasmid

    #158077

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPU-huMDA5-miR30-MDA5

    Plasmid

    #174224

    Purpose

    All-in-one huMDA5 rescue-MDA5 shRNA knockdown vector

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MDA5 (IFIH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

    Plasmid

    #63885

    Purpose

    An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized low-affinity GCaMP3(10.19)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPRISM-TagRFP-gcry-eGFP

    Plasmid

    #117767

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MBP-rPKM2 WT

    Plasmid

    #107158

    Purpose

    MBP-rPKM2 WT for production and purification of rat PKM2 WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase M2 isoform (Pkm Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-GFP-IMP2-2_antisense-control

    Plasmid

    #42174

    Depositor

    Alexandra Kiemer

    Article

    Kessler et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid

    #114087

    Purpose

    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for FnCas12a crRNAs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

    Plasmid

    #136660

    Purpose

    Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    ABE7.10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS and hSt1Cas9

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR1A-HA-Jag1

    Plasmid

    #46052

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only