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pFB-MBD2.6R (pc2397)

Plasmid

#211725

Purpose

Expresses MBD2 (aa153-235) RFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.6 aa153-235

Tags

RFP

Expression

Insect

Promoter

Polyhedrin promotor

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.1mR (pc2391)

Plasmid

#211723

Purpose

Expresses MBD2 (aa1-152) RFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.1 aa1-152

Tags

RFP

Expression

Insect

Promoter

Polyhedrin promotor

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB_MBD2.2-GFP (pc2083)

Plasmid

#211722

Purpose

Expresses MBD2 (aa153-414) GFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.2 aa153-414

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB_MBD2.1-GFP (pc2082)

Plasmid

#211721

Purpose

Expresses MBD2 (aa1-152) GFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2 aa1-152

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB MBD2a-2-strep (pc1794)

Plasmid

#211715

Purpose

Expresses fl. MBD2 2xStrep tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Tags

2xStrep

Expression

Insect

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFB MBD2a-C-GFP (pc1565)

Plasmid

#211712

Purpose

Expresses fl. MBD2 GFP tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-hXRCC1pd-HIS

Plasmid

#206036

Purpose

Bacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoter

Depositor

Primo Schaer

Publication

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

HIS

Expression

Bacterial

Mutation

S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

Promoter

T7

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:TDimer mVenus

Plasmid

#210520

Purpose

Expresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Publication

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Tandem dimer mVenus

Use

Zebrafish expression, tol2 kit gateway-compatible

Promoter

Zebrafish insulin promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:R198P mVenus

Plasmid

#210519

Purpose

Expresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Publication

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Apollo-NADP+

Use

Zebrafish expression, tol2 kit gateway-compatible

Tags

mVenus

Mutation

R198P

Promoter

Zebrafish insulin promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEG302-TRB2-ZF

Plasmid

#200926

Purpose

Express TRB2-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Publication

Wang et al Nat Commun. 2023 Mar 28;14(1):1736. doi:

Insert

TRB2 (TRB2 Mustard Weed)

Expression

Plant

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

Plasmid

#189634

Purpose

Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

Depositor

Manuel Kaulich

Publication

Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

Insert

hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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hPRL2_1-163

Plasmid

#209794

Purpose

Bacterial expression of human PRL-2 without farnesylation motif

Depositor

Kalle Gehring

Publication

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-2 (PTP4A2 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-163 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-shBACH1_BFP

Plasmid

#199217

Purpose

Expression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone.

Depositor

Gabor Balazsi

Publication

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

shBACH1 (BACH1 Synthetic)

Use

RNAi

Promoter

human U6

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GALL-Bat-CoV-HKU9_3CL

Plasmid

#203478

Purpose

Expresses Bat-CoV HKU9 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Publication

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-CoV HKU9 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-HNRNPD_sgRNA2

Plasmid

#201599

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Publication

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HNRNPD (HNRNPD Human)

Use

CRISPR and Lentiviral

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PABPC1_sgRNA2

Plasmid

#201609

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Publication

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PABPC1 (PABPC1 Human)

Use

CRISPR and Lentiviral

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-NAD-NLS3x

Plasmid

#193822

Purpose

CMV overexpression of ChemoG-NAD in the nucleus of mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-NAD-NLS3x

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-ChemoL-NAD-NLS3x

Plasmid

#193828

Purpose

CMV overexpression of ChemoL-NAD in the nucleus of mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-NAD-NLS3x

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD

Plasmid

#193829

Purpose

CMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

[4xCox8]-ChemoL-NAD

Expression

Mammalian

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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vYM010_phiC31-nTRE-pEF1s(high)-H2b-Citrine

Plasmid

#197678

Purpose

"pEF1s(high)" reporter plasmid

Depositor

Michael Elowitz

Publication

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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