We narrowed to 1,414 results for: puc57

1. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175508

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

   Plasmid

   #175509

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. ppk301-T2A-QF2 HDR plasmid

   Plasmid

   #130667

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
   

   Use

   Donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-hEF1A-MG-U6-sgRNA

   Plasmid

   #175505

   Purpose

   All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF1a, U6

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CT3G-CERP2-MG-IRES2-mCherry

   Plasmid

   #175507

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVH1002-Tier2(pUC ori)

   Plasmid

   #169600

   Purpose

   Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGS1021

   Plasmid

   #59575

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
   

   Use

   E. coli cloning vector

   Tags

   Twin-Strep tag

   Expression

   Mutation

   Promoter

   None

   Available since

   Aug. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175506

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPotef-cdc3CB16-NatR

   Plasmid

   #86465

   Purpose

   Expresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.

   Depositor

   Michael Feldbrügge

   Article

   Baumann et al EMBO Rep. 2014 Jan;15(1):94-102. doi: 10

   Insert

   Cdc3
   

   Use

   Ustilago maydis expression

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Potef

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2356_Tier3(PB)-PuroR

    Plasmid

    #169655

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGS1034

    Plasmid

    #63796

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1

    Insert

    CBP-3xFLAG-TEV-Protein A followed by KlLEU2
    

    Use

    E. coli cloning vector

    Tags

    CBP-3xFLAG-TEV-Protein A

    Expression

    Mutation

    Promoter

    none

    Available since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVH617

    Plasmid

    #169606

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGS1022

    Plasmid

    #59576

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Twin-Strep tag

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-APEX2-mCherry

    Plasmid

    #188944

    Purpose

    Middle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry

    Depositor

    Owen Tamplin

    Article

    Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

    Insert

    mito-APEX2_p2A_APEX2-H2B_p2A_mCherry
    

    Use

    Middle entry vector for gateway (l1-l2)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH615

    Plasmid

    #169604

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAVS1 Insulator ObLiGaRe/EPB71

    Plasmid

    #90018

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by insulator and inverted ZFN binding sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGS1011

    Plasmid

    #63783

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Strep-tag II followed by TRP1 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS2371

    Plasmid

    #225637

    Purpose

    High Copy number plasmid containing Ple388 (PITX3 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

    Insert

    Ple388 (PITX3 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS2341_Tier3(SB)-Puro

    Plasmid

    #169639

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVH616

    Plasmid

    #169605

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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