We narrowed to 5,988 results for: crispr cas9 expression plasmids

1. pCRISPomyces-2

   Plasmid

   #61737

   Purpose

   Streptomyces expression of codon-optimized Cas9 and custom gRNA

   Depositor

   Huimin Zhao

   Article

   Cobb et al ACS Synth Biol. 2014 Dec 8.

   Inserts

   sSpCas9
   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   BbsI-flanked lacZ cassette inserted in place of s…

   Promoter

   gapdhp(EL) and rpsL(XC)-BbsI

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. GE-0010-gRNA2-MpCKB

   Plasmid

   #238528

   Purpose

   Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta

   Depositor

   Michael Hothorn

   Insert

   MpCKB
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GE-0010-gRNA1-MpCKB

   Plasmid

   #238527

   Purpose

   Plant expression of Cas9 and gRNA against M. polymorpha CK2 beta

   Depositor

   Michael Hothorn

   Insert

   MpCKB
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GE-0010-gRNA1-MpCKA

   Plasmid

   #238526

   Purpose

   Plant expression of Cas9 and gRNA against M. polymorpha CK2 alpha

   Depositor

   Michael Hothorn

   Insert

   MpCKA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPTG-GG-sg2+sg3

   Plasmid

   #127199

   Purpose

   Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA
   

   Use

   Empty sgrna plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PTG

   Plasmid

   #127198

   Purpose

   Single template U6 promoter minimal size plasmid

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Type

   Empty backbone

   Use

   Empty sgrna plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TLCV2

   Plasmid

   #87360

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-2A-eGFP
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Tags

   Cas9-T2A-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW1328

   Plasmid

   #69488

   Purpose

   sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

   Depositor

   Jordan Ward

   Insert

   sgRNA(F+E) targeting pha-1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   R07E5.16 U6 promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEasyG1_nat

    Plasmid

    #184911

    Purpose

    Template to generate via PCR a single gRNA for expression in S. cerevisiae

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pATF4.1.0-gDNA

    Plasmid

    #113772

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4

    Depositor

    Kevin White

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pELK3.1.0-gDNA

    Plasmid

    #112418

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

    Depositor

    Kevin White

    Insert

    ELK3 (ELK3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJUNB.1.0-gDNA

    Plasmid

    #112409

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB

    Depositor

    Kevin White

    Insert

    JUNB (JUNB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pESR1.1.0-gDNA

    Plasmid

    #112433

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1

    Depositor

    Kevin White

    Insert

    ESR1 (ESR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTFE3.1.0-gDNA

    Plasmid

    #112473

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3

    Depositor

    Kevin White

    Insert

    TFE3 (TFE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pERF.1.0-gDNA

    Plasmid

    #112395

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERF

    Depositor

    Kevin White

    Insert

    ERF (ERF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBCL6.1.0-gDNA

    Plasmid

    #112485

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6

    Depositor

    Kevin White

    Insert

    BCL6 (BCL6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pE2F3.3.1-gDNA

    Plasmid

    #113776

    Purpose

    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor E2F3

    Depositor

    Kevin White

    Insert

    E2F3 (E2F3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCREB1.1.0-gDNA

    Plasmid

    #113770

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CREB1

    Depositor

    Kevin White

    Insert

    CREB1 (CREB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCSDC2.1.0-gDNA

    Plasmid

    #113781

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CSDC2

    Depositor

    Kevin White

    Insert

    CSDC2 (CSDC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCLOCK.1.0-gDNA

    Plasmid

    #112406

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CLOCK

    Depositor

    Kevin White

    Insert

    CLOCK (CLOCK Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFOXO4.1.0-gDNA

    Plasmid

    #112408

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXO4

    Depositor

    Kevin White

    Insert

    FOXO4 (FOXO4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDEAF1.2.2-gDNA

    Plasmid

    #113799

    Purpose

    CRISPR plasmid for expression of Cas9 nicakse (D10A) and gRNA targeting human transcription factor DEAF1

    Depositor

    Kevin White

    Insert

    DEAF1 (DEAF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHOXC13.1.0-gDNA

    Plasmid

    #113795

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HOXC13

    Depositor

    Kevin White

    Insert

    HOXC13 (HOXC13 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pISL1.1.0-gDNA

    Plasmid

    #113788

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ISL1

    Depositor

    Kevin White

    Insert

    ISL1 (ISL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEHF.1.0-gDNA

    Plasmid

    #113785

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor EHF

    Depositor

    Kevin White

    Insert

    EHF (EHF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFOXF2.1.0-gDNA

    Plasmid

    #113784

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXF2

    Depositor

    Kevin White

    Insert

    FOXF2 (FOXF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHOXB5.1.0-gDNA

    Plasmid

    #113783

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HOXB5

    Depositor

    Kevin White

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHOXB13.1.0-gDNA

    Plasmid

    #113782

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HOXB13

    Depositor

    Kevin White

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGLIS3.1.0-gDNA

    Plasmid

    #113780

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor GLIS3

    Depositor

    Kevin White

    Insert

    GLIS3 (GLIS3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSRF.1.0-gDNA

    Plasmid

    #112458

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SRF

    Depositor

    Kevin White

    Insert

    SRF (SRF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDEAF1.2.1-gDNA

    Plasmid

    #112449

    Purpose

    CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor DEAF1

    Depositor

    Kevin White

    Insert

    DEAF1 (DEAF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pE2F5.1.0-gDNA

    Plasmid

    #112447

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5

    Depositor

    Kevin White

    Insert

    E2F5 (E2F5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDLX1.1.0-gDNA

    Plasmid

    #112439

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DLX1

    Depositor

    Kevin White

    Insert

    DLX1 (DLX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHBP1.1.0-gDNA

    Plasmid

    #112432

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HBP1

    Depositor

    Kevin White

    Insert

    HBP1 (HBP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pATF1.1.0-gDNA

    Plasmid

    #112424

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF1

    Depositor

    Kevin White

    Insert

    ATF1 (ATF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRG2

    Plasmid

    #104174

    Purpose

    sgRNA expression

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. HCP5

    Plasmid

    #166107

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.

    Depositor

    Matthias Heinemann

    Insert

    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge