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Showing: 2151 - 2200 of 18908 results

  1. loxP-T2A-Myr-DeadCasp8-ERT2-rox

    Plasmid
    #182361
    Purpose
    Shuttle vector for T2A-Myr-DeadCasp8-ERT2
    Depositor
    Mario Capecchi
    Insert
    loxP-T2A-Myr-DeadCasp8-ERT2-rox
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182361-plasmid-map-sequence-id-358216

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  2. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid
    #135669
    Purpose
    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    sgPten
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135669-plasmid-map-sequence-id-325841

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  3. CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

    Plasmid
    #172477
    Purpose
    sfGFP with a synthetic intron derived from the human beta-globin gene
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172477-plasmid-map-sequence-id-338915

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  4. CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

    Plasmid
    #172478
    Purpose
    sfGFP with a synthetic intron from the pCI expression vector (Promega)
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172478-plasmid-map-sequence-id-338912

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  5. CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron

    Plasmid
    #172475
    Purpose
    sfGFP with a synthetic intron derived from the mouse Ighe gene
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172475-plasmid-map-sequence-id-338910

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  6. CAG-loxP-3xpA-loxP-sfGFP+RbG syntron

    Plasmid
    #172476
    Purpose
    sfGFP with a synthetic intron derived from the rabbit beta-globin gene
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172476-plasmid-map-sequence-id-338906

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  7. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid
    #135391
    Purpose
    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.
    Depositor
    Fan Wang
    Insert
    TeLC-P2A-EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSynapsin
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    135391-plasmid-map-sequence-id-266854

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  8. pCambia-loxKan

    Plasmid
    #199590
    Purpose
    basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility
    Depositor
    Andreas Stuermer
    Insert
    FRT-lox66-NosP-KanR-NosT-lox71-FRT
    Use
    Cre/Lox
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Nos
    Available since
    July 5, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199590-plasmid-map-sequence-id-399425

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  9. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid
    #200661
    Purpose
    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina
    Depositor
    Samer Hattar
    Insert
    DREADD Gq-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    200661-plasmid-map-sequence-id-393501

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  10. AAV-DIO-TVA-2A-oG

    Plasmid
    #178431
    Purpose
    Expresses TVA and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950
    Optimized G
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178431-plasmid-map-sequence-id-354735

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  11. pAAV-hSyn-con/fon DREADD Gi-mCherry

    Plasmid
    #200660
    Purpose
    Construct for chemogenetically silencing and visualizing subpopulations of neurons in the retina
    Depositor
    Samer Hattar
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    200660-plasmid-map-sequence-id-393500

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  14. pLP-mOlig2-ires-gfp

    Plasmid
    #49399
    Purpose
    mammalian bicistronic expression of mouse Olig2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Olig2 (Olig2 Mouse)
    Use
    Cre/Lox; Acceptor vector
    Tags
    EGFP and IRES
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    49399-plasmid-map-sequence-id-316898

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  15. AAV-DIO-TC66T-2A-oG

    Plasmid
    #178430
    Purpose
    Expresses TVA(TC66T) and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950 Glu66→Thr
    Optimized G
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178430-plasmid-map-sequence-id-354734

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  16. p159-RV-syn-dio-mScarlet-T2A

    Plasmid
    #185700
    Purpose
    Retroviral vector expression cre-dependent membrane tagged mScarlet-T2A driven by the rat Synapsin promoter (with XhoI and PacI at 3'end of T2A to clone a gene of interest)
    Depositor
    Lynette Lim
    Insert
    mScarlet
    Use
    Retroviral
    Tags
    mScarlet palmitoylation with T2A on 3'-end
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185700-plasmid-map-sequence-id-366334

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  17. pBSIIKS_HIS3_I

    Plasmid
    #109062
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109062-plasmid-map-sequence-id-209541

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  18. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

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  22. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162075-plasmid-map-sequence-id-325304

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  29. iChr2-Notch Mosaic (SO250)

    Plasmid
    #99752
    Purpose
    Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genes
    Depositor
    Rui Benedito
    Article
    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do
    Insert
    HIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99752-plasmid-map-sequence-id-182454

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  32. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104492
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104492-plasmid-map-sequence-id-381181

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  33. pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE

    Plasmid
    #104496
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV PHP.eB, AAV Retrograde, and AAV1
    Insert
    jGCaMP7f
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only

  34. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

    Plasmid
    #104493
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV Retrograde and AAV1
    Insert
    jGCaMP7b
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104493-plasmid-map-sequence-id-200290

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  38. Lenti-LucAL

    Plasmid
    #174049
    Purpose
    Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucAL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine; LAMA4 glycine 1254…
    Promoter
    Human Ubiquitin C
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    174049-plasmid-map-sequence-id-345204

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  39. pEMS1132

    Plasmid
    #29053
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP/Cre fusion reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-Cre-NLS
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2012
    Availability
    Academic Institutions and Nonprofits only
    29053-plasmid-map-sequence-id-13468

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  40. Lenti-LucA

    Plasmid
    #174047
    Purpose
    Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucA
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    ALG8 alanine 506 to threonine
    Promoter
    Human Ubiquitin C
    Available since
    Availability
    Academic Institutions and Nonprofits only
    174047-plasmid-map-sequence-id-345203

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  41. Lenti-LucL

    Plasmid
    #174048
    Purpose
    Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinase
    Depositor
    Tyler Jacks
    Article
    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.
    Insert
    LucL
    Use
    Cre/Lox, Lentiviral, and Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    LAMA4 glycine 1254 to valine
    Promoter
    Human Ubiquitin C
    Available since
    Availability
    Academic Institutions and Nonprofits only
    174048-plasmid-map-sequence-id-345139

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  42. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51083
    Purpose
    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51083-plasmid-map-sequence-id-175189

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  44. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51082
    Purpose
    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51082-plasmid-map-sequence-id-175187

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  45. pAAV hSyn-Flex RG-cerulean

    Plasmid
    #98221
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under hSyn promoter, for producing AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV
    Tags
    cerulean
    Expression
    Mutation
    Promoter
    human Synapsin
    Available since
    Aug. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    98221-plasmid-map-sequence-id-192715

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  46. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid
    #172479
    Purpose
    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172479-plasmid-map-sequence-id-338913

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  48. Ef1a-DIO-somAion-citrine

    Plasmid
    #192573
    Purpose
    AAV-vector for optogenetic long-term silencing of Cre-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somAion
    Use
    AAV
    Tags
    Citrine - soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Mutation
    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…
    Promoter
    Ef1-alpha
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    192573-plasmid-map-sequence-id-379368

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Showing: 2151 - 2200 of 18908 results