We narrowed to 2,230 results for: D10

1. TFORF3538

   Plasmid

   #145014

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HOXD10 (HOXD10 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Target-AID (pRZ762)

   Plasmid

   #131300

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

   Plasmid

   #185915

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201819

   Purpose

   A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Expression

   Mammalian

   Promoter

   pOTTC1751

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-nSpCas9-TadA8e

   Plasmid

   #209044

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8e (ABE8e) is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8e-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

   Plasmid

   #140004

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EFS-SaKKH-spA-miniU6-miniSdd6-MmHPD-T2

   Plasmid

   #204852

   Purpose

   AAV vector for cytosine base editing using miniSdd6 at HPD-T2 target site in mouse

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd6-nSaCas9KKH(D10A)-UGI
   

   Use

   AAV and CRISPR

   Mutation

   D10A, E782K, N968K, R1015H in SaCas9

   Promoter

   EFS, miniU6

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pdCas9 (GB1079)

   Plasmid

   #75399

   Purpose

   Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. attB-GFP-Tet1d834-1363-Poly(A)

   Plasmid

   #65560

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locus

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   del(834-1363)

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human Wild-type ASAP1

    Plasmid

    #235212

    Purpose

    Expresses Wild-Type ASAP1 in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Tsai et al Proc Natl Acad Sci U S A. 2018 May 1;115

    Insert

    ASAP1 (ASAP1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

    Plasmid

    #177344

    Purpose

    AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    dead hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3x GCN4 peptide, 3xHA, and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    Tetracycline-dependent promoters

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-nSpCas9-TadA8.20m

    Plasmid

    #209045

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TadA8.20m-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-EDAR-COMP5AP-AviTag-9xHis

    Plasmid

    #157119

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EDAR (EDAR Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid

    #140003

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid

    #185911

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid

    #185916

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-nSpCas9-evoCDA1

    Plasmid

    #209042

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    evoCDA1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid

    #197504

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_PEX3_WT

    Plasmid

    #81779

    Purpose

    Gateway Donor vector containing PEX3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PEX3 (PEX3 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-nSpCas9-APOBEC3A

    Plasmid

    #209040

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC3A-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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